ENPP1 Polyclonal antibody

ENPP1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 14011-1-AP

Synonyms

E NPP 1, ENPP1, M6S1, PC 1, PC1, PCA1, PDNP1

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Validation Data Gallery

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  WB analysis of Raji using 14011-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 14011-1-AP (ENPP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of Raji using 14011-1-AP

  IP result of anti-ENPP1 (IP:14011-1-AP, 4ug; Detection:14011-1-AP 1:1000) with Raji cells lysate 3200 ug.

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Tested Applications

• Positive WB detected in: Raji cells
• Positive IP detected in: Raji cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3200
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14011-1-AP targets ENPP1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ENPP1 fusion protein Ag5117
• Full Name: ectonucleotide pyrophosphatase/phosphodiesterase 1
• Calculated Molecular Weight: 105 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC059375
• Gene Symbol: ENPP1
• Gene ID (NCBI): 5167
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.