ENPP4 Polyclonal antibody

ENPP4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 17305-1-AP

Synonyms

E NPP 4, ENPP4, KIAA0879

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Validation Data Gallery

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  WB analysis of HL-60 using 17305-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 17305-1-AP (ENPP4 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 17305-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 17305-1-AP (ENPP4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 17305-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17305-1-AP (ENPP4 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 17305-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17305-1-AP (ENPP4 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: HL-60 cells
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

17305-1-AP targets ENPP4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ENPP4 fusion protein Ag11192
• Full Name: ectonucleotide pyrophosphatase/phosphodiesterase 4 (putative function)
• Calculated Molecular Weight: 453 aa, 52 kDa
• Observed Molecular Weight: 52-60 kDa
• GenBank Accession Number: BC018054
• Gene Symbol: ENPP4
• Gene ID (NCBI): 22875
• RRID: AB_2099780
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ENPP4, also named as KIAA0879 or NPP4, is a 453 amino acid protein, which belongs to the nucleotide pyrophosphatase/phosphodiesterase family. ENPP4 localizes in the cell membrane and hydrolyzes extracellular Ap3A into AMP and ADP, and Ap4A into AMP and ATP. ENPP4 is expressed on the surface of vascular endothelia.

Publications

Species	Application	Title
human	IHC	Blood; NPP4 is a procoagulant enzyme on the surface of vascular endothelium.; Authors - Albright Ronald A RA