ENTPD2 Polyclonal antibody

ENTPD2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 13656-1-AP

Synonyms

CD39 antigen like 1, CD39L1, Ecto ATP diphosphohydrolase 2, Ecto ATPase 2, Ecto ATPDase 2, ENTPD2, NTPDase 2

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Validation Data Gallery

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  WB analysis of human placenta using 13656-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 13656-1-AP (ENTPD2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 13656-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13656-1-AP (ENTPD2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 13656-1-AP

  IP result of anti-ENTPD2 (IP:13656-1-AP, 4ug; Detection:13656-1-AP 1:300) with K-562 cells lysate 4000ug.

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  IHC staining of human small intestine using 13656-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 13656-1-AP (ENTPD2 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human small intestine using 13656-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 13656-1-AP (ENTPD2 antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: human placenta tissue, K-562 cells
• Positive IP detected in: K-562 cells
• Positive IHC detected in: human small intestine tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

13656-1-AP targets ENTPD2 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ENTPD2 fusion protein Ag4513
• Full Name: ectonucleoside triphosphate diphosphohydrolase 2
• Calculated Molecular Weight: 495 aa, 54 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC035738
• Gene Symbol: ENTPD2
• Gene ID (NCBI): 954
• RRID: AB_2877966
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ENTPD2(Ectonucleoside triphosphate diphosphohydrolase 2) is also named as CD39L1, Ecto-ATPase 2 and belongs to the GDA1/CD39 NTPase family. In the nervous system, it could hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. This protein has 3 isoforms produced by alternative splicing.

Publications

Species	Application	Title
rat	IHC	Am J Physiol Gastrointest Liver Physiol; Water intake accelerates ATP release from myofibroblast cells in rats: ATP-mediated podoplanin-dependent control for physiological function and immunity.; Authors - Moyuru Hayashi