p300 Polyclonal antibody

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• p300 Polyclonal antibody (20695-1-AP)

• 

  WB analysis using 20695-1-AP

  Various lysates were subjected to Tris-Acetate gel system followed by western blot with 20695-1-AP (p300 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 20695-1-AP

  Various lysates were subjected to Tris-Acetate gel system followed by western blot with 20695-1-AP (p300 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

p300 Polyclonal Antibody for WB, ELISA

Cat No. 20695-1-AP

Publications(29)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, IP, CoIP, ChIP, ELISA

Synonyms

EP300, E1A-associated protein p300, EC:2.3.1.-, EC:2.3.1.48, Histone butyryltransferase p300

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Tested Applications

• Positive WB detected in: HEK-293T cells, MCF-7 cells, HeLa cells, Jurkat cells, RAW 264.7 cells, PC-3 cells

It is recommended to use NETN lysis buffer for sample preparation during WB detection.

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 28 publications below
• IHC: See 4 publications below
• IF: See 2 publications below
• IP: See 1 publications below
• CoIP: See 3 publications below
• ChIP: See 1 publications below

Product Information

20695-1-AP targets p300 in WB, IHC, IF, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: E1A binding protein p300
• Calculated Molecular Weight: 264 kDa
• Observed Molecular Weight: 300 kDa
• GenBank Accession Number: NM_001429
• Gene Symbol: EP300
• Gene ID (NCBI): 2033
• RRID: AB_3085614
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q09472
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

p300 is a 300 kDa global transcriptional coactivator with histone acetyltransferase (HAT) activity. p300 binds to chromosomal histones and modulates the chromatin conformation by acetylation of its histones, leading to activation of various gene transcriptions. p300 plays a critical role in DNA repair synthesis after DNA damage induced by ultraviolet or radiation. p300 is overexpressed in various types of cancer, which is associated with worse patient outcomes (PMID: 27322077).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Commun Biol; Exosome-derived circ-001422 promotes tumor-associated macrophage M2 polarization to accelerate the progression of glioma; Authors - Wenpeng Cao
human	WB	Inflammation; Narciclasine Alleviates Endothelial Inflammation and Atherosclerosis Initiation by Inhibiting Histone Lactylation-Mediated NF-κB Activation.; Authors - Ziqian Wang
human,mouse	WB	Int J Biol Sci; Curcumol Induces Necroptosis of Hepatic Stellate Cells by Targeting KAT8 to Suppress HK2 Lactylation and Promote HUWE1-Dependent Ubiquitination.; Authors - Xiaohan Guo
human	WB,IF	Adv Sci (Weinh); PIK3CA Mutations Downregulate PPT1 to Promote Adipogenesis by Suppressing P300 Depalmitoylation and Phase Separation.; Authors - Hongrui Chen
human	WB,CoIP	iScience; KLF13-mediated CES2 upregulation via p300-dependent acetylation sensitizes gastric cancer cells to irinotecan.; Authors - Hai-bin Zhang
mouse	WB	Research (Wash D C); Vascular Smooth Muscle Cell-Specific BCAT2 Deficiency Attenuates Diabetic Atherosclerotic Calcification via Histone Propionylation.; Authors - Lili Zhang