p300 Polyclonal antibody

p300 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 20695-1-AP

Synonyms

E1A associated protein p300, E1A binding protein p300, EP300, Histone acetyltransferase p300, KAT3B, p300, p300 HAT

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Validation Data Gallery

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  WB analysis using 20695-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20695-1-AP (p300 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 20695-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20695-1-AP (p300 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 20695-1-AP

  Various lysates were subjected to Tris-acetate gel system followed by western blot with 20695-1-AP (p300 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse lung using 20695-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 20695-1-AP (p300 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse lung using 20695-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 20695-1-AP (p300 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat lung using 20695-1-AP

  Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 20695-1-AP (p300 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293T cells, MCF-7 cells, Jurkat cells, PC-3 cells
• Positive IHC detected in: rat lung tissue, mouse lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

20695-1-AP targets p300 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: E1A binding protein p300
• Calculated Molecular Weight: 264 kDa
• Observed Molecular Weight: 300 kDa
• GenBank Accession Number: NM_001429
• Gene Symbol: EP300
• Gene ID (NCBI): 2033
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

p300 is a 300 kDa global transcriptional coactivator with histone acetyltransferase (HAT) activity. p300 binds to chromosomal histones and modulates the chromatin conformation by acetylation of its histones, leading to activation of various gene transcriptions. p300 plays a critical role in DNA repair synthesis after DNA damage induced by ultraviolet or radiation. p300 is overexpressed in various types of cancer, which is associated with worse patient outcomes (PMID: 27322077).

Publications

Species	Application	Title
human	WB	Cell Metab; Targeting gut microbial nitrogen recycling and cellular uptake of ammonium to improve bortezomib resistance in multiple myeloma; Authors - Yinghong Zhu