Tested Applications
| Positive WB detected in | HL-60 cells, Jurkat cells |
| Positive IP detected in | HL-60 cells |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 7 publications below |
| IF | See 1 publications below |
| IP | See 1 publications below |
Product Information
13014-1-AP targets EPB41 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4075 Product name: Recombinant human EPB41 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-299 aa of BC039079 Sequence: MTTEKSLVTEAENSQHQQKEEGEEAINSGQQEPQQEESCQTAAEGDNWCEQKLKASNGDTPTHEDLTKNKERTSESRGLSRLFSSFLKRPKSQVSEEEGKEVESDKEKGEGGQKEIEFGTSLDEEIILKAPIAAPEPELKTDPSLDLHSLSSAETQPAQEELREDPDFEIKEGEGLEECSKIEVKEESPQSKAETELKASQKPIRKHRNMHCKVSLLDDTVYECVVEKHAKGQDLLKRVCEHLNLLEEDYFGLAIWDNATSKTWLDSAKEIKKQVRGVPWNFTFNVKFYPPDPAQLTED Predict reactive species |
| Full Name | erythrocyte membrane protein band 4.1 (elliptocytosis 1, RH-linked) |
| Calculated Molecular Weight | 720 aa, 81 kDa |
| Observed Molecular Weight | 80 kDa, 135 kDa |
| GenBank Accession Number | BC039079 |
| Gene Symbol | EPB41 |
| Gene ID (NCBI) | 2035 |
| RRID | AB_2098362 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P11171 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for EPB41 antibody 13014-1-AP | Download protocol |
| IP protocol for EPB41 antibody 13014-1-AP | Download protocol |
| WB protocol for EPB41 antibody 13014-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Cortical Dynein and asymmetric membrane elongation coordinately position the spindle in anaphase.
| ||
EMBO J NuMA interacts with phosphoinositides and links the mitotic spindle with the plasma membrane. | ||
Front Oncol Identification of a Two-Gene (PML-EPB41) Signature With Independent Prognostic Value in Osteosarcoma. | ||
Int Immunopharmacol Protein 4.1R regulates M1 macrophages polarization via glycolysis, alleviating sepsis-induced liver injury in mice | ||
Immunology Cytoskeleton Protein 4.1R Regulates B cell fate by Modulating the Canonical NF-κB Pathway.
|
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Himanshi (Verified Customer) (07-28-2025) | the antibody worked well in 5% skim milk powder blocking buffer
|
