EPB41 Polyclonal antibody

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• EPB41 Polyclonal antibody (13014-1-AP)

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• KD/KO Validated

EPB41 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 13014-1-AP Copied!

Publications (9)

Reviews (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Synonyms

Erythrocyte membrane protein band 4.1, EPB4.1, EPB 41, Band 4.1, 4.1R

• 

  WB analysis using 13014-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13014-1-AP (EPB41 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HL-60 using 13014-1-AP

  IP result of anti-EPB41 (IP:13014-1-AP, 4ug; Detection:13014-1-AP 1:200) with HL-60 cells lysate 4000ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human stomach using 13014-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13014-1-AP (EPB41 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HL-60 cells, Jurkat cells
• Positive IP detected in: HL-60 cells
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13014-1-AP targets EPB41 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4075

  Product name: Recombinant human EPB41 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-299 aa of BC039079

  Sequence: MTTEKSLVTEAENSQHQQKEEGEEAINSGQQEPQQEESCQTAAEGDNWCEQKLKASNGDTPTHEDLTKNKERTSESRGLSRLFSSFLKRPKSQVSEEEGKEVESDKEKGEGGQKEIEFGTSLDEEIILKAPIAAPEPELKTDPSLDLHSLSSAETQPAQEELREDPDFEIKEGEGLEECSKIEVKEESPQSKAETELKASQKPIRKHRNMHCKVSLLDDTVYECVVEKHAKGQDLLKRVCEHLNLLEEDYFGLAIWDNATSKTWLDSAKEIKKQVRGVPWNFTFNVKFYPPDPAQLTED

• Full Name: erythrocyte membrane protein band 4.1 (elliptocytosis 1, RH-linked)
• Calculated Molecular Weight: 720 aa, 81 kDa
• Observed Molecular Weight: 80 kDa, 135 kDa
• GenBank Accession Number: BC039079
• Gene Symbol: EPB41
• Gene ID (NCBI): 2035
• RRID: AB_2098362
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P11171
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The antibody is anti-4.1R (EPB41), catalog number 13014-1-AP, with 9 total citations. These appear in journals including Cell, EMBO Journal, Cells, International Immunopharmacology, Immunology, RNA, Frontiers in Medicine, Virus Research, and Frontiers in Oncology. Research fields span cell biology and mitotic spindle positioning, cytoskeletal regulation, B-cell and immune signaling, red blood cell membrane/skeleton, viral entry, and cancer prognosis.

Automatically generated from the 9 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cell; Cortical Dynein and asymmetric membrane elongation coordinately position the spindle in anaphase.; Authors - Kiyomitsu Tomomi T ; KD Validated
human	WB	EMBO J; NuMA interacts with phosphoinositides and links the mitotic spindle with the plasma membrane.; Authors - Sachin Kotak
		Cells; Cytoskeletal Protein 4.1R Inhibits BCR-Mediated B-Cell Activation by Restraining AKT1 Phosphorylation.; Authors - Yuying Guo
	WB,IF	Int Immunopharmacol; Protein 4.1R regulates M1 macrophages polarization via glycolysis, alleviating sepsis-induced liver injury in mice; Authors - Si-Yao Sang
mouse	WB	Immunology; Cytoskeleton Protein 4.1R Regulates B cell fate by Modulating the Canonical NF-κB Pathway.; Authors - Taotao Liang ; KO Validated
human,mouse	IP	RNA; 7SL RNA in Vertebrate Red Blood Cells.; Authors - Gaëlle J S Talhouarne

Reviews

5.0 out of 5

1 verified customer reviews

What customers say

The single review for this antibody is highly positive, rated 5 stars. The user tested it on K562 cells for Western Blot at a 1:1000 dilution and reported that the antibody worked well when used with a 5% skim milk powder blocking buffer. Overall, the feedback indicates reliable performance in WB applications.

Automatically generated from the 1 verified customer reviews below by Able AI.

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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Himanshi (Verified Customer) (07-28-2025)

the antibody worked well in 5% skim milk powder blocking buffer

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: k562