Dematin Polyclonal antibody

Dematin Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 16808-1-AP

Synonyms

Dematin, DMT, DMTN, EPB49

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Validation Data Gallery

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  WB analysis using 16808-1-AP

  human red blood cells were subjected to SDS PAGE followed by western blot with 16808-1-AP (Dematin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 16808-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 16808-1-AP (Dematin antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: rat brain tissue, human red blood cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

16808-1-AP targets Dematin in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Dematin fusion protein Ag10300
• Full Name: erythrocyte membrane protein band 4.9 (dematin)
• Calculated Molecular Weight: 405 aa, 46 kDa
• Observed Molecular Weight: 48-52 kDa
• GenBank Accession Number: BC017445
• Gene Symbol: EPB49
• Gene ID (NCBI): 2039
• RRID: AB_2277891
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Dematin (band 4.9) is a widely expressed cytoskeletal protein which regulates cell shape and cell migration. It is best known for its role within the red blood cell (RBC), where it has been localized to the spectrin-actin junctions of RBC membrane. Dematin has a 48 kDa subunit containing a headpiece domain and a 52 kDa subunit.

Publications

Species	Application	Title
human	WB	Front Med; Facile discovery of red blood cell deformation and compromised membrane/skeleton assembly in Prader-Willi syndrome; Authors - Yashuang Yang
human,mouse	WB,IHC,IF	Exp Cell Res; Dematin inhibits glioblastoma malignancy through RhoA-mediated CDKs downregulation and cytoskeleton remodeling.; Authors - Mengying Wang