EPHA1-specific Monoclonal antibody, PBS Only

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  WB analysis of HeLa using 60155-2-Ig (same clone as 60155-2-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 60155-2-Ig (EPHA1-specific antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60155-2-PBS in a different storage buffer formulation.

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  WB analysis of COLO 320 using 60155-2-Ig (same clone as 60155-2-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 60155-2-Ig (EPHA1-specific antibody at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60155-2-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 60155-2-Ig (same clone as 60155-2-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 60155-2-Ig (EPHA1-specific antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60155-2-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 60155-2-Ig (same clone as 60155-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60155-2-Ig (EPHA1-specific antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60155-2-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 60155-2-Ig (same clone as 60155-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60155-2-Ig (EPHA1-specific antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60155-2-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 60155-2-Ig (same clone as 60155-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60155-2-Ig (EPHA1-specific antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60155-2-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 60155-2-Ig (same clone as 60155-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60155-2-Ig (EPHA1-specific antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60155-2-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 60155-2-Ig (same clone as 60155-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60155-2-Ig (EPHA1-specific antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60155-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 60155-2-Ig (same clone as 60155-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60155-2-Ig (EPHA1-specific antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60155-2-PBS in a different storage buffer formulation.

EPHA1-specific Monoclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 60155-2-PBS

Clone No.1B7C3

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

EPH, EPH receptor A1, EPHA1, EPHA1-specific, Ephrin type A receptor 1, EPHT, EPHT1

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Product Information

60155-2-PBS targets EPHA1-specific in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: EPH receptor A1
• Calculated Molecular Weight: 108 kDa
• Observed Molecular Weight: 108 kDa
• GenBank Accession Number: BC130291
• Gene Symbol: EPHA1
• Gene ID (NCBI): 2041
• RRID: AB_10646478
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Caprylic acid/ammonium sulfate precipitation
• UNIPROT ID: P21709
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

EPHA1, also named as EPH, EPHT and EPHT1, belongs to ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. Ligands in the ephrin-A subclass, including the prototype family member ephrin-A1 (B61), are membrane associated through glycosylphosphatidyl-inositol linkages, whereas ephrin-B subclass consists of ligands with transmembrane domains. The general role of the Eph family is in mediating repulsive cell-cell interaction.