EPHA2 Polyclonal antibody

EPHA2 Polyclonal Antibody for WB, ELISA
Cat No. 31469-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

EC:2.7.10.1, ECK, EPH receptor A2, Ephrin type A receptor 2, Ephrin type-A receptor 2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inA431 cells, A549 cells, Calu-1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31469-1-AP targets EPHA2 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EPHA2 fusion protein Eg0146 Predict reactive species
Full Name EPH receptor A2
Calculated Molecular Weight 976 aa, 108 kDa
Observed Molecular Weight125-130 kDa
GenBank Accession NumberBC037166
Gene Symbol EPHA2
Gene ID (NCBI) 1969
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP29317
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EPHA2 (Ephrin type-A receptor 2) belongs to the receptor tyrosine kinase (RTK) family, with 16 known receptors and 9 known membrane-bound ligands in all species. Based on the extracellular domain sequence homology, structure, and binding affinity, Eph receptors and their ephrin ligands can be divided into A and B subtypes (PMID: 33962882). EPHA2 contains a conserved N-terminal ligand-bound extracellular domain, a transmembrane domain, and a conserved tyrosine kinase domain (PMID: 8070404).

Protocols

Product Specific Protocols
WB protocol for EPHA2 antibody 31469-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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