EPHB4 Polyclonal antibody

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• EPHB4 Polyclonal antibody (20883-1-AP)

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• KD/KO Validated

EPHB4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 20883-1-AP

Publications(29)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

EC:2.7.10.1, EPH receptor B4, Ephrin type B receptor 4, Ephrin type-B receptor 4, HTK

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  WB analysis using 20883-1-AP

  HT-29 cells were subjected to SDS PAGE followed by western blot with 20883-1-AP (EPHB4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  WB analysis of MCF-7 using 20883-1-AP

  WB result of EPHB4 antibody (20883-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EPHB4 transfected MCF-7 cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 20883-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20883-1-AP (EPHB4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HT-1080 using 20883-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 20883-1-AP (EPHB4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of DU 145 using 20883-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 20883-1-AP (EPHB4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of PC-3 using 20883-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 20883-1-AP (EPHB4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human lung cancer using 20883-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20883-1-AP (EPHB4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human lung cancer using 20883-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20883-1-AP (EPHB4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human pancreas using 20883-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 20883-1-AP (EPHB4 antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human pancreas using 20883-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 20883-1-AP (EPHB4 antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human placenta using 20883-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 20883-1-AP (EPHB4 antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human lung cancer using 20883-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 20883-1-AP (EPHB4 antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human placenta using 20883-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 20883-1-AP (EPHB4 antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human lung cancer using 20883-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 20883-1-AP (EPHB4 antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HT-29 using 20883-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HT-29 cells using EPHB4 antibody (20883-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HT-29 cells, DU 145 cells, HeLa cells, HT-1080 cells, MCF-7 cells, PC-3 cells
• Positive IHC detected in: human lung cancer tissue, human pancreas tissue, human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HT-29 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20883-1-AP targets EPHB4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag10033

  Product name: Recombinant human EPHB4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 639-987 aa of BC052804

  Sequence: KKESCVAIKTLKGGYTERQRREFLSEASIMGQFEHPNIIRLEGVVTNSMPVMILTEFMENGALDSFLRLNDGQFTVIQLVGMLRGIASGMRYLAEMSYVHRDLAARNILVNSNLVCKVSDFGLSRFLEENSSDPTYTSSLGGKIPIRWTAPEAIAFRKFTSASDAWSYGIVMWEVMSFGERPYWDMSNQDVINAIEQDYRLPPPPDCPTSLHQLMLDCWQKDRNARPRFPQVVSALDKMIRNPASLKIVARENGGASHPLLDQRQPHYSAFGSVGEWLRAIKMGRYEESFAAAGFGSFELVSQISAEDLLRIGVTLAGHQKKILASVQHMKSQAKPGTPGGTGGPAPQY

• Full Name: EPH receptor B4
• Calculated Molecular Weight: 987 aa, 108 kDa
• Observed Molecular Weight: 110-120 kDa
• GenBank Accession Number: BC052804
• Gene Symbol: EPHB4
• Gene ID (NCBI): 2050
• RRID: AB_10897158
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P54760
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EPHB4 (Ephrin type-B receptor 4) is a member of the Eph receptor tyrosine kinase family, the largest group of receptor tyrosine kinases. It binds ephrin-B ligands to regulate embryonic vascular development, lymphangiogenesis, and adult angiogenesis. Aberrant EPHB4 signaling promotes tumor progression, metastasis, and therapy resistance in various cancers, including breast, prostate, and colorectal malignancies. As a key regulator of tumor vasculature and the tumor microenvironment, EPHB4 represents an emerging therapeutic target for anti-cancer drug development and a potential prognostic biomarker.

Publications

What published studies show

Automatically generated from the publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	IF	Small; Specific Photothermal Ablation Therapy of Endometriosis by Targeting Delivery of Gold Nanospheres.; Authors - Xiaomeng Guo
human	IF	Drug Deliv; Near infrared light mediated photochemotherapy for efficiently treating deep orthotopic tumors guided by ultrasound imaging.; Authors - Zuhua Wang
	WB,IHC	J Cell Physiol; Intermittent parathyroid hormone promotes cementogenesis via ephrinB2-EPHB4 forward signaling.; Authors - Tiancheng Li
human	WB	J Cell Mol Med; Kinome multigenic panel identified novel druggable EPHB4-V871I somatic variant in high-risk neuroblastoma.; Authors - Immacolata Andolfo
human		Phytomedicine; Sanguinarine combats hypoxia-induced activation of EphB4 and HIF-1α pathways in breast cancer.; Authors - Qi Su
rat	IHC	Commun Biol; The application of tissue-engineered fish swim bladder vascular graft.; Authors - Hualong Bai

Reviews

5.0 out of 5

1 verified customer reviews

What customers say

Automatically generated from the verified customer reviews below by Able AI.

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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Marsilius (Verified Customer) (04-13-2021)

This antibody is useful for staining.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: brain

EPHB4 Antibody Immunofluorescence validation (1:100 dilution) in brain (Cat no:20883-1-AP)