Anti-Human EPHB4 Rabbit Recombinant Antibody, PBS Only

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• Anti-Human EPHB4 Rabbit Recombinant Antibody, PBS Only (98685-3-PBS)

EPHB4 Uni-rAb^® Recombinant Antibody for FC

Cat No. 98685-3-PBS

Clone No.252186C2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

EC:2.7.10.1, EPH receptor B4, Ephrin type B receptor 4, Ephrin type-B receptor 4, HTK

• 

  FC experiment of MCF-7 using 98685-3-RR (same clone as 98685-3-PBS)

  1x10^6 MCF-7 cells were surface stained with 0.25 ug Anti-Human EPHB4 Rabbit RecAb (98685-3-RR, Clone:252186C2) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were not fixed. This data was developed using the same antibody clone with 98685-3-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Affinity of 98685-3-RR

  Biolayer interferometry (BLl) kinetic assays of 98685-3-RR against Human EPHB4 were performed. The affinity constant is 1.45 nM.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Product Information

98685-3-PBS targets EPHB4 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg6504

  Product name: Recombinant Human EPHB4 protein (rFc Tag)

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 16-539 aa of NM_004444.4

  Sequence: LEETLLNTKLETADLKWVTFPQVDGQWEELSGLDEEQHSVRTYEVCDVQRAPGQAHWLRTGWVPRRGAVHVYATLRFTMLECLSLPRAGRSCKETFTVFYYESDADTATALTPAWMENPYIKVDTVAAEHLTRKRPGAEATGKVNVKTLRLGPLSKAGFYLAFQDQGACMALLSLHLFYKKCAQLTVNLTRFPETVPRELVVPVAGSCVVDAVPAPGPSPSLYCREDGQWAEQPVTGCSCAPGFEAAEGNTKCRACAQGTFKPLSGEGSCQPCPANSHSNTIGSAVCQCRVGYFRARTDPRGAPCTTPPSAPRSVVSRLNGSSLHLEWSAPLESGGREDLTYALRCRECRPGGSCAPCGGDLTFDPGPRDLVEPWVVVRGLRPDFTYTFEVTALNGVSSLATGPVPFEPVNVTTDREVPPAVSDIRVTRSSPSSLSLAWAVPRAPSGAVLDYEVKYHEKGAEGPSSVRFLKTSENRAELRGLKRGASYLVQVRARSEAGYGPFGQEHHSQTQLDESEGWREQLA

• Full Name: EPH receptor B4
• Calculated Molecular Weight: 108 kDa
• GenBank Accession Number: NM_004444.4
• Gene Symbol: EPHB4
• Gene ID (NCBI): 2050
• RRID: AB_3746273
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P54760
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

EPHB4 (Ephrin type-B receptor 4), a member of the largest family of receptor tyrosine kinases (RTK), is overexpressed in several tumor types, including prostate, breast, and bladder tumors and down-regulation of EPHB4 in tumor cell lines of these origins results in reduced cell viability, migration, and invasion. In endothelial cells, where EphB4 is normally expressed, it regulates cell migration, proliferation, and invasion (PMID: 15930280). The discovery of EphB4 as an alternative Epo receptor has multiple clinical implications including opportunities for patient stratification and anti-EphB4 approaches to abrogate the stimulatory effects of Epo on tumor growth (PMID: 16982731, 16424904).