EPHX2 Recombinant monoclonal antibody

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• EPHX2 Recombinant monoclonal antibody (86790-1-RR)

• 

  WB analysis using 86790-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86790-1-RR (EPHX2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse colon using 86790-1-RR

  IP result of anti-EPHX2 (IP:86790-1-RR, 4ug; Detection:86790-1-RR 1:600) with mouse colon tissue lysate 1480 ug.

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  Affinity and Kinetic Characterization of 86790-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86790-1-RR against Human EPHX2 were performed. The affinity constant is below 1 pM.

EPHX2 Uni-rAb^® Recombinant Antibody for WB, IP, ELISA

Cat No. 86790-1-RR

Clone No.251753F4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, ELISA

Synonyms

Bifunctional epoxide hydrolase 2, CEH, Cytosolic epoxide hydrolase 2, Lipid-phosphate phosphatase, SEH

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Tested Applications

• Positive WB detected in: U-937 cells, HEK-293 cells, A549 cells, LNCaP cells, Jurkat cells, mouse colon tissue
• Positive IP detected in: mouse colon tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86790-1-RR targets EPHX2 in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag1283

  Product name: Recombinant human EPHX2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 212-555 aa of BC013874

  Sequence: ELEKVTGIQLLNTPAPLPTSCNPSDMSHGYVTVKPRVRLHFVELGSGPAVCLCHGFPESWYSWRYQIPALAQAGYRVLAMDMKGYGESSAPPEIEEYCMEVLCKEMVTFLDKLGLSQAVFIGHDWGGMLVWYMALFYPERVRAVASLNTPFIPANPNMSPLESIKANPVFDYQLYFQEPGVAEAELEQNLSRTFKSLFRASDESVLSMHKVCEAGGLFVNSPEEPSLSRMVTEEEIQFYVQQFKKSGFRGPLNWYRNMERNWKWACKSLGRKILIPALMVTAEKDFVLVPQMSQHMEDWIPHLKRGHIEDCGHWTQMDKPTEVNQILIKWLDSDARNPPVVSKM

• Full Name: epoxide hydrolase 2, cytoplasmic
• Calculated Molecular Weight: 63 kDa
• Observed Molecular Weight: 57 kDa
• GenBank Accession Number: BC013874
• Gene Symbol: EPHX2
• Gene ID (NCBI): 2053
• RRID: AB_3745119
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P34913
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EPHX2(Epoxide hydrolase 2) acts on epoxides (alkene oxides, oxiranes) and arene oxides and plays a role in xenobiotic metabolism by degrading potentially toxic epoxides. A number of single nucleotide polymorphisms (SNPs) in human EPHX2 have been linked to cardiovascular disease risk, including increased risk of coronary heart disease, hyperlipoproteinemia, and type-2 diabetes (PMID: 14732757, 16595607, 14673705, 15845398, 17460077). It was observed in many tissues with the band of 63 kDa in the western blot. It has also been reported that the N-terminal domain might promote dimerization of EPHX2 (PMID:21553642). Negative expression of EPHX2 protein in U-937 cells is consistent with the predicted expression pattern.