EPM2A/Laforin Polyclonal antibody

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• EPM2A/Laforin Polyclonal antibody (33843-1-AP)

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  WB analysis using 33843-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 33843-1-AP (EPM2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 33843-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 33843-1-AP (EPM2A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse skeletal muscle using 33843-1-AP

  IP result of anti-EPM2A (IP:33843-1-AP, 4ug; Detection:33843-1-AP 1:800) with mouse skeletal muscle tissue lysate 1360 ug.

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  IP experiment of HL-60 using 33843-1-AP

  IP result of anti-EPM2A (IP:33843-1-AP, 4ug; Detection:33843-1-AP 1:800) with HL-60 cells lysate 1480 ug.

EPM2A/Laforin Polyclonal Antibody for WB, IP, ELISA

Cat No. 33843-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Synonyms

EPM2A, Laforin, EC:3.1.3.48, Glucan phosphatase, Glycogen phosphatase

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Tested Applications

• Positive WB detected in: HL-60 cells, Ramos cells, rat skeletal muscle tissue
• Positive IP detected in: HL-60 cells, mouse skeletal muscle tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

33843-1-AP targets EPM2A/Laforin in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag36983

  Product name: Recombinant human EPM2A protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-331 aa of BC070047

  Sequence: MRFRFGVVVPPAVAGARPELLVVGSRPELGRWEPRGAVRLRPAGTAAGDGALALQEPGLWLGEVELAAEEAAQDGAEPGRVDTFWYKFLKREPGGELSWEGNGPHHDRCCTYNENNLVDGVYCLPIGHWIEATGHTNEMKHTTDFYFNIAGHQAMHYSRILPNIWLGSCPRQVEHVTIKLKHELGITAVMNFKTEWDIVQNSSGCNRYPEPMTPDTMIKLYREEGLAYIWMPTPDMSTEGRVQMLPQAVCLLHALLEKGHIVYVHCNAGVGRSTAAVCGWLQYVMGWNLRKVQYFLMAKRPAVYIDEEALARAQEDFFQKFGKVRSSVCSL

• Full Name: epilepsy, progressive myoclonus type 2A, Lafora disease (laforin)
• Calculated Molecular Weight: 37 kDa
• Observed Molecular Weight: 37 kDa
• GenBank Accession Number: BC070047
• Gene Symbol: EPM2A
• Gene ID (NCBI): 7957
• RRID: AB_3743091
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: O95278
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EPM2A encodes the glucan phosphatase laforin, a type of dual specificity phosphatase, and EPM2B encodes malin, an E3-ubiquitin ligase (PMID: 22364389). Laforin is encoded by the 130 Kb four-exon gene EPM2A on chromosome 6q24 of the human genome. It is ubiquitously expressed in all tissues, although brain, skeletal muscle, heart and liver have higher levels of expression (PMID: 9931343). Laforin has also been shown to interact with other regulatory subunits of PP1, including GL and R6, members of the same family as protein targeting to glycogen (PTG) (PMID: 27702709). In the brain, laforin is expressed predominantly in cerebellum, hippocampus, frontal cortex and olfactory bulb. Laforin expression increases after birth, reaching a maximum during the adulthood (PMID: 11355878). EPM2A alternative splicing results in two laforin isoforms that are identical from amino acid 1-309, but contain a divergent C-terminal domain. Isoform laforin-331 is the most abundant form, possesses phosphatase activity, and when overexpressed in cell culture localizes to the cytoplasm and ER (PMID: 18617530).