EPN1 Polyclonal antibody

EPN1 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 14469-1-AP

Synonyms

EPN1, EPS 15 interacting protein 1, epsin 1

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Validation Data Gallery

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  WB analysis using 14469-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14469-1-AP (EPN1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 14469-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using EPN1 antibody (14469-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A431 cells, HEK-293 cells, HeLa cells, K-562 cells
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14469-1-AP targets EPN1 in WB, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EPN1 fusion protein Ag5838
• Full Name: epsin 1
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: BC044651
• Gene Symbol: EPN1
• Gene ID (NCBI): 29924
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.