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HER2/ErbB2 Polyclonal antibody
HER2/ErbB2 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
WB, IP, IHC, IF, FC, ELISA
Conjugate
Unconjugated
Cat no : 18299-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | 4T1 cells, SGC-7901 cells, MCF-7 cells, HeLa cells |
| Positive IP detected in | SGC-7901 cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | human breast cancer tissue |
| Positive FC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 40 publications below |
| IHC | See 12 publications below |
| IF | See 3 publications below |
| FC | See 1 publications below |
Product Information
18299-1-AP targets HER2/ErbB2 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide |
| Full Name | v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) |
| Calculated molecular weight | 138 kDa |
| Observed molecular weight | 185 kDa |
| GenBank accession number | BC167147 |
| Gene symbol | ERBB2 |
| Gene ID (NCBI) | 2064 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
HER2, also known as ErbB2 and Neu, is a 185-kDa transmembrane glycoprotein that is a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. It has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Amplification and/or overexpression of HER2 have been reported in numerous cancers, including breast and ovarian tumors. HER2 is a therapeutic target for the treatment of breast cancer and other carcinomas. This antibody raised against a synthesized peptide corresponding to 1237-1255aa of human HER2 recognizes the 185-kDa full-length glycosylated form and other lower molecular-mass forms of HER2, including the truncated form.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for HER2/ErbB2 antibody 18299-1-AP | Download protocol |
| IHC protocol for HER2/ErbB2 antibody 18299-1-AP | Download protocol |
| IF protocol for HER2/ErbB2 antibody 18299-1-AP | Download protocol |
| IP protocol for HER2/ErbB2 antibody 18299-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Res Protein C receptor is a therapeutic stem cell target in a distinct group of breast cancers. | ||
Small Efficient Delivery of GSDMD-N mRNA by Engineered Extracellular Vesicles Induces Pyroptosis for Enhanced Immunotherapy | ||
Nat Commun Targeting 17q23 amplicon to overcome the resistance to anti-HER2 therapy in HER2+ breast cancer. | ||
J Immunother Cancer Genome-scale CRISPR-Cas9 screen reveals novel regulators of B7-H3 in tumor cells. | ||
Cancer Res Disrupting Circadian Rhythm via the PER1-HK2 Axis Reverses Trastuzumab Resistance in Gastric Cancer. | ||
ACS Sens Rolling Circle Amplification-Assisted Flow Cytometry Approach for Simultaneous Profiling of Exosomal Surface Proteins. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Vanina (Verified Customer) (07-28-2021) | MDA-MB-453 cell line was transfected with different concentrations of HER2/Erbb2 siRNA for 48h. Western blot using HER2/Erbb2 rabbit Proteintech antibody (18299-1-AP) at 1:2,000 dillution confirms the HER2/Erbb2 knockdown.
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