ERBB2IP Polyclonal antibody

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  WB analysis using 22438-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22438-1-AP (ERBB2IP antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 22438-1-AP

  WB result of ERBB2IP antibody (22438-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ERBB2IP transfected MCF-7 cells.

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  IHC staining of human kidney using 22438-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 22438-1-AP (ERBB2IP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 22438-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 22438-1-AP (ERBB2IP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of H9C2 using 22438-1-AP

  Immunofluorescent analysis of (4% PFA) fixed H9C2 cells using ERBB2IP antibody (22438-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• Featured Product
• KD/KO Validated

ERBB2IP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 22438-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

LAP2, KIAA1225, ERBIN, Erbb2-interacting protein, Densin-180-like protein

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Tested Applications

• Positive WB detected in: HeLa cells, MCF-7 cells
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: H9C2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

22438-1-AP targets ERBB2IP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ERBB2IP fusion protein Ag18206
• Full Name: erbb2 interacting protein
• Calculated Molecular Weight: 1412 aa, 158 kDa
• Observed Molecular Weight: 150-180 kDa
• GenBank Accession Number: BC115012
• Gene Symbol: ERBB2IP
• Gene ID (NCBI): 55914
• RRID: AB_2879105
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96RT1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ERBB2IP is a cytoplasmic protein that contains leucine-rich repeats (LRR) and PSD95/Dlg1/zo-1 (PDZ) domain (thus named a LAP protein). ERBB2IP interacts, via its single PDZ domain, specifically with ErbB2, but not with ErbB3, ErbB4, or EGFR. It is colocalized with ErbB2 at the basolateral membranes of epithelial cells. In addition, ERBB2IP also interacts with PSD‐95, Lano and with three p120‐related catenins, eBPAG1 and the β4 subunit of the α6β4 integrin, two major components of hemidesmosomes. The molecular weight of ERBB2IP was predicted 150~180 kDa by Western Blot.(PMID: 25288731,PMID: 24252174,PMID: 19289088)

Publications

Species	Application	Title
		J Exp Med; ERBIN deficiency links STAT3 and TGF-β pathway defects with atopy in humans.; Authors - J J Lyons
human	WB,IHC	Oncol Lett; Reduced IκBα promotes hepatocellular carcinoma cell proliferation and migration via regulation of NF-κB/Erbin axis.; Authors - Ye Tan
human	WB	Mol Cancer Res; Desmoglein 1 regulates invadopodia by suppressing EGFR/Erk signaling in an Erbin-dependent manner.; Authors - Alejandra Valenzuela-Iglesias