ERBB2IP Polyclonal antibody

ERBB2IP Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF

Conjugate

Unconjugated

Publications

3

Cat no : 22438-1-AP

Synonyms

Densin 180 like protein, erbb2 interacting protein, ERBB2IP, ERBIN, KIAA1225, LAP2, Protein LAP2

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Validation Data Gallery

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  WB analysis of MCF-7 using 22438-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 22438-1-AP (ERBB2IP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 22438-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22438-1-AP (ERBB2IP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 22438-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 22438-1-AP (ERBB2IP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 22438-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 22438-1-AP (ERBB2IP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 22438-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 22438-1-AP (ERBB2IP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 22438-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 22438-1-AP (ERBB2IP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: MCF7 cells, HeLa cells, MCF-7 cells
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

The immunogen of 22438-1-AP is ERBB2IP Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ERBB2IP fusion protein Ag18206
• Full Name: erbb2 interacting protein
• Calculated molecular weight: 1412 aa, 158 kDa
• Observed molecular weight: 150-180 kDa
• GenBank accession number: BC115012
• Gene symbol: ERBB2IP
• Gene ID (NCBI): 55914
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Publications

Species	Application	Title
human	WB	Mol Cancer Res; Desmoglein 1 regulates invadopodia by suppressing EGFR/Erk signaling in an Erbin-dependent manner.; Authors - Alejandra Valenzuela-Iglesias
human	WB,IHC	Oncol Lett; Reduced IκBα promotes hepatocellular carcinoma cell proliferation and migration via regulation of NF-κB/Erbin axis.; Authors - Ye Tan
		J Exp Med; ERBIN deficiency links STAT3 and TGF-β pathway defects with atopy in humans.; Authors - J J Lyons