ERC2 Polyclonal antibody

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• ERC2 Polyclonal antibody (21396-1-AP)

ERC2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 21396-1-AP Copied!

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

ERC protein 2, KIAA0378

• 

  WB analysis using 21396-1-AP

  The fetal human brain was subjected to SDS PAGE followed by western blot with 21396-1-AP (ERC2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human bowen disease using 21396-1-AP

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 21396-1-AP (ERC2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human bowen disease using 21396-1-AP

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 21396-1-AP (ERC2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 21396-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ERC2 antibody (21396-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: fetal human brain tissue
• Positive IHC detected in: human bowen disease; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21396-1-AP targets ERC2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag16080

  Product name: Recombinant human ERC2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 768-827 aa of BC111550

  Sequence: LKHNQQLEKKKNAQLLEEVRRREDSMADNSQHLQIEELMNALEKTRQELDATKARLASTQ

• Full Name: ELKS/RAB6-interacting/CAST family member 2
• Calculated Molecular Weight: 957 aa, 111 kDa
• Observed Molecular Weight: 115-120 kDa
• GenBank Accession Number: BC111550
• Gene Symbol: ERC2
• Gene ID (NCBI): 26059
• RRID: AB_10859782
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O15083
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The ERC2 polyclonal antibody (Cat# 21396-1-AP) has 1 citation to date. It appears in Translational Psychiatry (2025), in a study investigating how G6PD deficiency in the brain induces schizophrenia-like behaviors and synaptic dysfunction in mice. The research field is neuroscience/psychiatry, specifically related to neurodevelopmental disorders and synaptic function. The antibody was used for Western blot in this publication.

Automatically generated from the 1 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Transl Psychiatry; G6PD deficiency in brain induces schizophrenia-like behaviors and synaptic dysfunction.; Authors - Yu-Bing Wang