ERC2 Polyclonal antibody

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  WB analysis of human brain using 21396-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 21396-1-AP (ERC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 21396-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 21396-1-AP (ERC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human bowen disease using 21396-1-AP

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 21396-1-AP (ERC2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human bowen disease using 21396-1-AP

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 21396-1-AP (ERC2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 21396-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ERC2 antibody (21396-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

ERC2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 21396-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

KIAA0378, ERC protein 2

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Tested Applications

• Positive WB detected in: human brain tissue, rat brain tissue
• Positive IHC detected in: human bowen disease; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21396-1-AP targets ERC2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag16080

  Product name: Recombinant human ERC2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 768-827 aa of BC111550

  Sequence: LKHNQQLEKKKNAQLLEEVRRREDSMADNSQHLQIEELMNALEKTRQELDATKARLASTQ

• Full Name: ELKS/RAB6-interacting/CAST family member 2
• Calculated Molecular Weight: 957 aa, 111 kDa
• Observed Molecular Weight: 115-120 kDa
• GenBank Accession Number: BC111550
• Gene Symbol: ERC2
• Gene ID (NCBI): 26059
• RRID: AB_10859782
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O15083
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.