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ERG Polyclonal antibody

ERG Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 14356-1-AP

Synonyms

ERG, erg 3, p55, Transcriptional regulator ERG, Transforming protein ERG



Tested Applications

Positive WB detected inMCF-7 cells, HEK-293 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman prostate cancer tissue, mouse brain tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:600-1:2400
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

14356-1-AP targets ERG in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ERG fusion protein Ag5708
Full Name v-ets erythroblastosis virus E26 oncogene homolog (avian)
Calculated molecular weight 55 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC040168
Gene symbol ERG
Gene ID (NCBI) 2078
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ERG (ETS-related gene) is a member of the ETS family of transcription factors, which are downstream effectors of mitogenic signaling transduction pathway. ERG is a key regulator of cell proliferation, differentiation, angiogenesis, inflammation, and apoptosis. ERG is involved in chromosomal translocations, resulting in different fusion gene products, such as TMPSSR2-ERG and NDRG1-ERG in prostate cancer, EWS-ERG in Ewing's sarcoma and FUS-ERG in acute myeloid leukemia.

Protocols

Product Specific Protocols
WB protocol for ERG antibody 14356-1-APDownload protocol
IHC protocol for ERG antibody 14356-1-APDownload protocol
IF protocol for ERG antibody 14356-1-APDownload protocol
IP protocol for ERG antibody 14356-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Cancer

miR-223-5p targeting ERG inhibits prostate cancer cell proliferation and migration.

Authors - Yongbao Wei
  • KD Validated