Tested Applications
| Positive WB detected in | HepG2 cells, K-562 cells, MCF-7 cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16029-1-AP targets ERGIC3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8893 Product name: Recombinant human ERGIC3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 44-383 aa of BC009765 Sequence: SELQYYLTTEVHPELYVDKSRGDKLKINIDVLFPHMPCAYLSIDAMDVAGEQQLDVEHNLFKQRLDKDGIPVSSEAERHELGKVEVTVFDPDSLDPDRCESCYGAEAEDIKCCNTCEDVREAYRRRGWAFKNPDTIEQCRREGFSQKMQEQKNEGCQVYGFLEVNKVAGNFHFAPGKSFQQSHVHVHDLQSFGLDNINMTHYIQHLSFGEDYPGIVNPLDHTNVTAPQASMMFQYFVKVVPTVYMKVDGEVLRTNQFSVTRHEKVANGLLGDQGLPGVFVLYELSPMMVKLTEKHRSFTHFLTGVCAIIGGMFTVAGLIDSLIYHSARAIQKKIDLGKTT Predict reactive species |
| Full Name | ERGIC and golgi 3 |
| Calculated Molecular Weight | 383 aa, 43 kDa |
| Observed Molecular Weight | 50 kDa |
| GenBank Accession Number | BC009765 |
| Gene Symbol | ERGIC3 |
| Gene ID (NCBI) | 51614 |
| RRID | AB_2098446 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y282 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ERGIC3 (Endoplasmic reticulum-Golgi intermediate compartment protein 3 ) is located in the cis face of the Golgi apparatus and vesicular tubular structures between the transitional endoplasmic reticulum (ER) and cis-Golgi. ERGIC3 significantly affects cell growth and causes ER stress-induced cell death, and is involved in the invasion and metastasis in hepatocellular carcinomas (HCC). (PMID: 26177443, PMID: 31142615)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ERGIC3 antibody 16029-1-AP | Download protocol |
| IHC protocol for ERGIC3 antibody 16029-1-AP | Download protocol |
| WB protocol for ERGIC3 antibody 16029-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ERGIC3 Polyclonal antibody (Cat# 16029-1-AP) has 1 total citation. It appears in the Journal of Biological Chemistry (J Biol Chem), published in November 2021. The cited study focused on identifying TRAMs as sphingolipid-binding proteins using a photoactivatable and clickable short chain ceramide analog, placing the research in the fields of lipid metabolism and membrane protein biology. The application used was Western blot (WB) in human samples.
| Species | Application | Title |
|---|---|---|
J Biol Chem Identification of TRAMs as Sphingolipid-Binding Proteins Using a Photoactivatable and Clickable Short Chain Ceramide Analog. |
