ERMAP Polyclonal antibody

ERMAP Polyclonal Antibody for IF, IP,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 21375-1-AP

Synonyms

ERMAP, hERMAP, PRO2801, Radin blood group antigen, RD, SC, Scianna blood group antigen

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Validation Data Gallery

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  IP experiment of MCF-7 using 21375-1-AP

  IP result of anti-ERMAP (IP:21375-1-AP, 4ug; Detection:21375-1-AP 1:300) with MCF-7 cells lysate 1600ug.

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  IF Staining of MCF-7 using 21375-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 21375-1-AP (ERMAP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive IP detected in: MCF-7 cells
• Positive IF detected in: MCF-7 cells

Recommended dilution

• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

The immunogen of 21375-1-AP is ERMAP Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ERMAP fusion protein Ag15899
• Full Name: erythroblast membrane-associated protein (Scianna blood group)
• Calculated Molecular Weight: 475 aa, 53 kDa
• Observed Molecular Weight: 53 kDa
• GenBank Accession Number: BC099703
• Gene Symbol: ERMAP
• Gene ID (NCBI): 114625
• RRID: AB_2878849
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Nat Immunol; The ligation between ERMAP, galectin-9 and dectin-2 promotes Kupffer cell phagocytosis and antitumor immunity; Authors - Jie Li