TXNDC4 Polyclonal antibody

• Featured Product
• KD/KO Validated

TXNDC4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 16016-1-AP

Synonyms

ERp44, ER protein 44, UNQ532/PRO1075, Thioredoxin domain-containing protein 4, PDIA10

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Validation Data Gallery

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  WB analysis of HepG2 using 16016-1-AP

  WB result of TXNDC4 antibody (16016-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TXNDC4 transfected HepG2 cells.

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  WB analysis using 16016-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16016-1-AP (TXNDC4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 16016-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16016-1-AP (TXNDC4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16016-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16016-1-AP (TXNDC4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 16016-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 16016-1-AP (TXNDC4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16016-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16016-1-AP (TXNDC4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 16016-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 16016-1-AP (TXNDC4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 16016-1-AP

  IP result of anti-TXNDC4 (IP:16016-1-AP, 4ug; Detection:16016-1-AP 1:500) with K-562 cells lysate 4000ug.

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  IHC staining of human breast cancer using 16016-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16016-1-AP (TXNDC4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 16016-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16016-1-AP (TXNDC4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 16016-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 16016-1-AP (TXNDC4 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human breast cancer using 16016-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 16016-1-AP (TXNDC4 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HeLa using 16016-1-AP

  Immunofluorescent analysis of Hela cells, using ERP44 antibody 16016-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells, MCF-7 cells, K-562 cells
• Positive IP detected in: K-562 cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 3 publications below
• CoIP: See 1 publications below

Product Information

16016-1-AP targets TXNDC4 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TXNDC4 fusion protein Ag8818
• Full Name: endoplasmic reticulum protein 44
• Calculated Molecular Weight: 406 aa, 47 kDa
• Observed Molecular Weight: 44-47 kDa
• GenBank Accession Number: BC005374
• Gene Symbol: ERP44
• Gene ID (NCBI): 23071
• RRID: AB_1640025
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TXNDC4 is also named as KIAA0573, ERP44, PDIA10, ERp44 and belongs to the thioredoxin family (TRX). It may be involved in electron transport, protein folding, response to unfolded protein, glycoprotein metabolism, secretory pathway. It may have a role in the control of oxidative protein folding in the endoplasmic reticulum.

Publications

Species	Application	Title
human	IF	Cell Metab; Di-methylation of CD147-K234 Promotes the Progression of NSCLC by Enhancing Lactate Export.; Authors - Ke Wang
human	WB,IF	Antioxid Redox Signal; Ero1α regulates Ca(2+) fluxes at the endoplasmic reticulum-mitochondria interface (MAM).; Authors - Anelli Tiziana T
human	WB,IHC,CoIP	J Transl Med; ER resident protein 44 promotes malignant phenotype in nasopharyngeal carcinoma through the interaction with ATP citrate lyase.; Authors - Hui Tian ; KD Validated
human	WB	Traffic; Profound architectural and functional readjustments of the secretory pathway in decidualization of endometrial stromal cells.; Authors - Tiziana Anelli
human,mouse	WB,IHC	BMC Cancer; Exosomal ERp44 derived from ER-stressed cells strengthens cisplatin resistance of nasopharyngeal carcinoma; Authors - Tian Xia
human	WB,IF	Tissue Cell; Endoplasmic reticulum stress of endometrial mesenchymal stem cells in endometriosis; Authors - Selenay Furat Rencber