ESD Monoclonal antibody, PBS Only

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  WB analysis using 67069-2-Ig (same clone as 67069-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67069-2-Ig (ESD antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67069-2-PBS in a different storage buffer formulation.

• 

  IF Staining of HEK-293 using 67069-2-Ig (same clone as 67069-2-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using ESD antibody (67069-2-Ig, Clone: 2F5E1 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 67069-2-PBS in a different storage buffer formulation.

ESD Monoclonal Antibody for WB, IF/ICC, ELISA

Cat No. 67069-2-PBS

Clone No.2F5E1

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

Methylumbelliferyl-acetate deacetylase, FGH, Esterase D, EC:3.1.2.12, EC:3.1.1.56

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Product Information

67069-2-PBS targets ESD in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7487

  Product name: Recombinant human ESD protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-282 aa of BC001169

  Sequence: MALKQISSNKCFGGLQKVFEHDSVELNCKMKFAVYLPPKAETGKCPALYWLSGLTCTEQNFISKSGYHQSASEHGLVVIAPDTSPRGCNIKGEDESWDFGTGAGFYVDATEDPWKTNYRMYSYVTEELPQLINANFPVDPQRMSIFGHSMGGHGALICALKNPGKYKSVSAFAPICNPVLCPWGKKAFSGYLGTDQSKWKAYDATHLVKSYPGSQLDILIDQGKDDQFLLDGQLLPDNFIAACTEKKIPVVFRLQEDYDHSYYFIATFITDHIRHHAKYLNA

• Full Name: esterase D/formylglutathione hydrolase
• Calculated Molecular Weight: 31 kDa
• Observed Molecular Weight: 30-34 kDa
• GenBank Accession Number: BC001169
• Gene Symbol: ESD
• Gene ID (NCBI): 2098
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P10768
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Esterase D (ESD) is a non-specific esterase widely distributed in various organisms and is also named S-Formylglutathione Hydrolase (SFGH). ESD is a member of the carboxylesterase family and has both carboxylesterase and thioesterase activities. ESD plays an important role in the process of glutathione-dependent detoxification, regulating cholesterol efflux and virus infection in humans, and is closely related to the development of tumors. ESD as a Genetic Marker for Retinoblastoma (PMID: 32247735, PMID: 34875997, PMID: 35627173). The calculated molecular weight of ESD is 31 kDa.