ESR2 Beta 3 Polyclonal antibody

ESR2 Beta 3 Polyclonal Antibody for IF, IHC,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 55441-1-AP

Synonyms

ER BETA, Erb, ESR BETA, ESR2, ESR2 Beta 3, ESRB, ESTRB, estrogen receptor 2 (ER beta), Estrogen receptor beta, NR3A2



Tested Applications

Positive IHC detected inhuman cervix tissue, human breast cancer tissue, human endometrial cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

55441-1-AP targets ESR2 Beta 3 in IHC, IF,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name estrogen receptor 2 (ER beta)
Calculated molecular weight 58 kDa
GenBank accession numberNM_001437
Gene symbol ESR2
Gene ID (NCBI) 2100
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

Estrogen receptor-beta (ESR2) is a member of the superfamily of nuclear receptors, which can transduce extracellular signals into transcriptional responses. It binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual

Protocols

Product Specific Protocols
IHC protocol for ESR2 Beta 3 antibody 55441-1-APDownload protocol
IF protocol for ESR2 Beta 3 antibody 55441-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols