ETS2 Polyclonal antibody

ETS2 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 12280-1-AP

Synonyms

ETS2, ETS2IT1, Protein C ets 2

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Validation Data Gallery

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  WB analysis of K-562 using 12280-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12280-1-AP (ETS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 12280-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 12280-1-AP (ETS2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12280-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12280-1-AP (ETS2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 12280-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12280-1-AP (ETS2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 12280-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12280-1-AP (ETS2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 12280-1-AP

  IP result of anti-ETS2 (IP:12280-1-AP, 3ug; Detection:12280-1-AP 1:600) with HeLa cells lysate 5000ug.

• 

  IHC staining of mouse spleen using 12280-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 12280-1-AP (ETS2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 12280-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 12280-1-AP (ETS2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat liver using 12280-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 12280-1-AP (ETS2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat liver using 12280-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 12280-1-AP (ETS2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: K-562 cells, HeLa cells, MCF-7 cells, mouse kidney tissue, PC-3 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: mouse spleen tissue, rat liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

12280-1-AP targets ETS2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ETS2 fusion protein Ag2929
• Full Name: v-ets erythroblastosis virus E26 oncogene homolog 2 (avian)
• Calculated Molecular Weight: 469 aa, 53 kDa
• Observed Molecular Weight: 53 kDa
• GenBank Accession Number: BC017040
• Gene Symbol: ETS2
• Gene ID (NCBI): 2114
• RRID: AB_10804755
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Cell Death Dis; The overexpression of Tipe2 in CRC cells suppresses survival while endogenous Tipe2 accelerates AOM/DSS induced-tumor initiation.; Authors - Yan Li
human	WB	Cell Death Dis; A distal super-enhancer activates oncogenic ETS2 via recruiting MECOM in inflammatory bowel disease and colorectal cancer; Authors - Yongheng Chen