ETV5 Polyclonal antibody

• Featured Product
• KD/KO Validated

ETV5 Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, CoIP, ChIP,ELISA

Conjugate

Unconjugated

Publications

13

Cat no : 13011-1-AP

Synonyms

ERM, Ets related protein ERM, ETS translocation variant 5, ets variant 5, ETV5

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Validation Data Gallery

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  WB analysis using 13011-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13011-1-AP (ETV5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of PC-3 using 13011-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed PC-3 cells using 13011-1-AP (ETV5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, MCF-7 cells, PC-3 cells
• Positive IF detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 4 publications below
• WB: See 6 publications below
• IF: See 6 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Product Information

13011-1-AP targets ETV5 in WB, IF, CoIP, ChIP,ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ETV5 fusion protein Ag3708
• Full Name: ets variant 5
• Calculated molecular weight: 510 aa, 58 kDa
• Observed molecular weight: 58 kDa
• GenBank accession number: BC007333
• Gene symbol: ETV5
• Gene ID (NCBI): 2119
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ETS variant gene 5(ETV5) belongs to the ETS oncogene family that shares a conserved peptide, ETS domain, which mediates sequence-specific DNA binding. ETV5 is a transcription factor and is required for spermatogonial stem cell self-renewal. ETV5 can be negatively regulated by COP1, a tumor suppressor. Also it has a role in branching morphogenesis in the developing kidney

Publications

Species	Application	Title
mouse	WB	Mol Cell Endocrinol; ETV5 regulates GOAT/ghrelin system in an mTORC1-dependent manner.; Authors - Zhuo Mao ; KD Validated
mouse	IF	Dev Cell; FAT4 Fine-Tunes Kidney Development by Regulating RET Signaling.; Authors - Hongtao Zhang
mouse	WB,IF	Nat Commun; Capicua regulates neural stem cell proliferation and lineage specification through control of Ets factors.; Authors - Shiekh Tanveer Ahmad
mouse		BMC Biol; Biological and RNA regulatory function of MOV10 in mammalian germ cells.; Authors - Kaiqiang Fu
		Endocr Relat Cancer; Interaction of BRAF-induced ETS factors with mutant TERT promoter in papillary thyroid cancer.; Authors - Young Shin Song
human	WB	J Biol Chem; ETS variant transcription factor 5 and c-Myc cooperate in derepressing the human telomerase gene promoter via composite Ets/E-box motifs.; Authors - Fan Zhang ; KD Validated