ETV5 Polyclonal antibody

• Featured Product
• KD/KO Validated

ETV5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Publications

26

Cat no : 13011-1-AP

Synonyms

Ets-related protein ERM, ETS translocation variant 5, Ets related protein ERM, ERM

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Validation Data Gallery

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  WB analysis using 13011-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13011-1-AP (ETV5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13011-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13011-1-AP (ETV5 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 13011-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13011-1-AP (ETV5 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 13011-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13011-1-AP (ETV5 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of PC-3 using 13011-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed PC-3 cells using 13011-1-AP (ETV5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, MCF-7 cells, PC-3 cells, Raji cells
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 12 publications below
• IHC: See 1 publications below
• IF: See 11 publications below
• CoIP: See 1 publications below
• ChIP: See 5 publications below

Product Information

13011-1-AP targets ETV5 in WB, IHC, IF/ICC, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ETV5 fusion protein Ag3708
• Full Name: ets variant 5
• Calculated Molecular Weight: 510 aa, 58 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC007333
• Gene Symbol: ETV5
• Gene ID (NCBI): 2119
• RRID: AB_2278092
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ETS variant gene 5(ETV5) belongs to the ETS oncogene family that shares a conserved peptide, ETS domain, which mediates sequence-specific DNA binding. ETV5 is a transcription factor and is required for spermatogonial stem cell self-renewal. ETV5 can be negatively regulated by COP1, a tumor suppressor. Also it has a role in branching morphogenesis in the developing kidney

Publications

Species	Application	Title
human	WB	Cell; Loss of Ataxin-1 Potentiates Alzheimer's Pathogenesis by Elevating Cerebral BACE1 Transcription.; Authors - Jaehong Suh
human	ChIP	Nat Genet; The dynamic, combinatorial cis-regulatory lexicon of epidermal differentiation.; Authors - Daniel S Kim
mouse	WB	Nat Commun; A small secreted protein NICOL regulates lumicrine-mediated sperm maturation and male fertility; Authors - Daiji Kiyozumi
mouse	IF	Dev Cell; DDX20 is required for cell-cycle reentry of prospermatogonia and establishment of spermatogonial stem cell pool during testicular development in mice; Authors - Dingfeng Zou
mouse	IF	Nat Commun; A critical role for NF2 and the Hippo pathway in branching morphogenesis.; Authors - Antoine Reginensi
mouse	IF, ChIP	Proc Natl Acad Sci U S A; Transcription factor Etv5 is essential for the maintenance of alveolar type II cells.; Authors - Zhen Zhang