- Featured Product
- KD/KO Validated
ETV5 Polyclonal antibody
ETV5 Polyclonal Antibody for IF, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IF, CoIP, ChIP,ELISA
Conjugate
Unconjugated
Cat no : 13011-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, HepG2 cells, MCF-7 cells, PC-3 cells |
Positive IF detected in | PC-3 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 4 publications below |
WB | See 6 publications below |
IF | See 6 publications below |
CoIP | See 1 publications below |
ChIP | See 1 publications below |
Product Information
13011-1-AP targets ETV5 in WB, IF, CoIP, ChIP,ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | ETV5 fusion protein Ag3708 |
Full Name | ets variant 5 |
Calculated molecular weight | 510 aa, 58 kDa |
Observed molecular weight | 58 kDa |
GenBank accession number | BC007333 |
Gene symbol | ETV5 |
Gene ID (NCBI) | 2119 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ETS variant gene 5(ETV5) belongs to the ETS oncogene family that shares a conserved peptide, ETS domain, which mediates sequence-specific DNA binding. ETV5 is a transcription factor and is required for spermatogonial stem cell self-renewal. ETV5 can be negatively regulated by COP1, a tumor suppressor. Also it has a role in branching morphogenesis in the developing kidney
Protocols
Product Specific Protocols | |
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WB protocol for ETV5 antibody 13011-1-AP | Download protocol |
IF protocol for ETV5 antibody 13011-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Mol Cell Endocrinol ETV5 regulates GOAT/ghrelin system in an mTORC1-dependent manner.
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Nat Commun Capicua regulates neural stem cell proliferation and lineage specification through control of Ets factors. | ||
Endocr Relat Cancer Interaction of BRAF-induced ETS factors with mutant TERT promoter in papillary thyroid cancer. | ||
J Biol Chem ETS variant transcription factor 5 and c-Myc cooperate in derepressing the human telomerase gene promoter via composite Ets/E-box motifs.
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