ETV6 Polyclonal antibody

ETV6 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 13182-1-AP

Synonyms

ETS related protein Tel1, ETS translocation variant 6, ets variant 6, ETV6, TEL, TEL/ABL, TEL1, Transcription factor ETV6

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Validation Data Gallery

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  WB analysis using 13182-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13182-1-AP (ETV6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: A431 cells, HeLa cells, Jurkat cells, Raji cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13182-1-AP targets ETV6 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ETV6 fusion protein Ag3852
• Full Name: ets variant 6
• Calculated Molecular Weight: 452 aa, 53 kDa
• Observed Molecular Weight: 57-65 kDa
• GenBank Accession Number: BC043399
• Gene Symbol: ETV6
• Gene ID (NCBI): 2120
• RRID: AB_2246688
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ETV6, the E26 transformation-specific (ETS) family transcription repressor and tumor suppressor variant 6 gene, resides on chromosome 12p13, encodes a 57 kDa ETS family transcription factor with 3 highly conserved functional domains(PMID: 32841218). ETV6 is a well-studied hematopoietic transcription factor reported to play a key functional role in hematopoiesis, megakaryopoiesis, cell growth, and differentiation.