ETV6 Monoclonal antibody, PBS Only

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  WB analysis of Jurkat using 67579-1-Ig (same clone as 67579-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67579-1-Ig (ETV6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67579-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 67579-1-Ig (same clone as 67579-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 67579-1-Ig (ETV6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67579-1-PBS in a different storage buffer formulation.

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  WB analysis of THP-1 using 67579-1-Ig (same clone as 67579-1-PBS)

  THP-1 cells were subjected to SDS PAGE followed by western blot with 67579-1-Ig (ETV6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67579-1-PBS in a different storage buffer formulation.

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  WB analysis of U2OS using 67579-1-Ig (same clone as 67579-1-PBS)

  U2OS cells were subjected to SDS PAGE followed by western blot with 67579-1-Ig (ETV6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67579-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 67579-1-Ig (same clone as 67579-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 67579-1-Ig (ETV6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67579-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67579-1-Ig (same clone as 67579-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67579-1-Ig (ETV6 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67579-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 67579-1-Ig (same clone as 67579-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67579-1-Ig (ETV6 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67579-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 67579-1-Ig (same clone as 67579-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67579-1-Ig (ETV6 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67579-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 67579-1-Ig (same clone as 67579-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67579-1-Ig (ETV6 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67579-1-PBS in a different storage buffer formulation.

ETV6 Monoclonal Antibody for WB, IHC, ELISA

Cat No. 67579-1-PBS

Clone No.2A5A2

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, ELISA

Synonyms

ETS related protein Tel1, ETS translocation variant 6, ets variant 6, ETV6, TEL, TEL/ABL, TEL1, Transcription factor ETV6

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67579-1-Ig

Product Information

67579-1-PBS targets ETV6 in WB, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag30054

  Product name: Recombinant human ETV6 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 150-312 aa of BC043399

  Sequence: QNHEEDNCVQRTPRPSVDNVHHNPPTIELLHRSRSPITTNHRPSPDPEQRPLRSPLDNMIRRLSPAERAQGPRPHQENNHQESYPLSVSPMENNHCPASSESHPKPSSPRQESTRVIQLMPSPIMHPLILNPRHSVDFKQSRLSEDGLHREGKPINLSHREDL

• Full Name: ets variant 6
• Calculated Molecular Weight: 452 aa, 53 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC043399
• Gene Symbol: ETV6
• Gene ID (NCBI): 2120
• RRID: AB_2918493
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P41212
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

ETV6(ETS Variant Transcription Factor 6), also known as TEL, is a Protein Coding gene. ETV6 is a member of the ETS family of transcription factors encoded by the ETS variant 6 gene (ETV6), located on chromosome 12p13.2. ETV6 protein contains 3 domains: a) the pointed N-terminal (i.e. PNT) domain which forms oligomer partners with itself as well as other transcription factors (e.g. FLI1) and is required for ETV6's transcriptional repressing activity; b) the central regulatory domain; and c) the C-terminal DNA-binding domain(PMID: 22578774). ETV6 is highly expressed in early hematopoietic progenitor cells(HPCs) and is essential for hematopoiesis in the bone marrow(PMID: 31248877).