EVI5L Polyclonal antibody

• Home
• Products
• Primary Antibodies
• EVI5L Polyclonal antibody (32934-1-AP)

• 

  WB analysis using 32934-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32934-1-AP (EVI5L antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

EVI5L Polyclonal Antibody for WB, ELISA

Cat No. 32934-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

Ecotropic viral integration site 5-like protein, EVI5-like protein

---

Tested Applications

• Positive WB detected in: NIH/3T3 cells, RT-4 cells, U-251 cells, rat brain tissue, mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

32934-1-AP targets EVI5L in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag35996

  Product name: Recombinant human EVI5L protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 421-620 aa of BC014111

  Sequence: VVRQQCSSAAEDLQKAQSTIRQLQEQQENPRLTEDFVSHLETELEQSRLRETETLGALREMQDKVLDMEKRNSSLPDENNVAQLQEELKALKVREGQAVASTRELKLQLQELSDTWQAHLARGGRWKESPRKLVVGELQDELMSVRLREAQALAEGRELRQRVVELETQDHIHRNLLNRVEAERAALQEKLQYLAAQNKG

• Full Name: ecotropic viral integration site 5-like
• Observed Molecular Weight: 91,92 kDa
• GenBank Accession Number: BC014111
• Gene Symbol: EVI5L
• Gene ID (NCBI): 115704
• RRID: AB_3742796
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q96CN4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Evi5l (Ecotropic Viral Integration Site 5-Like), encodes a RabGAP that is known to block the formation of cilia when overexpressed (PMID: 26136363). EVI5L has ubiquitously low expression but is slightly higher in the testis and fetal brain.