Tested Applications
| Positive WB detected in | HEK-293T cells, Jurkat cells, HEK-293 cells, HeLa cells, Raji cells |
| Positive IP detected in | HEK-293T cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16253-1-AP targets EXO1/Exonuclease 1 in WB, IHC, IP, ChIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9253 Product name: Recombinant human EXO1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 496-846 aa of BC007491 Sequence: AVVVPGTRSRFFCSSDSTDCVSNKVSIQPLDETAVTDKENNLHESEYGDQEGKRLVDTDVARNSSDDIPNNHIPGDHIPDKATVFTDEESYSFESSKFTRTISPPTLGTLRSCFSWSGGLGDFSRTPSPSPSTALQQFRRKSDSPTSLPENNMSDVSQLKSEESSDDESHPLREGACSSQSQESGEFSLQSSNASKLSQCSSKDSDSEESDCNIKLLDSQSDQTSKLCLSHFSKKDTPLRNKVPGLYKSSSADSLSTTKIKPLGPARASGLSKKPASIQKRKHHNAENKPGLQIKLNELWKNFGFKKDSEKLPPCKKPLSPVRDNIQLTPEAEEDIFNKPECGRVQRAIFQ Predict reactive species |
| Full Name | exonuclease 1 |
| Calculated Molecular Weight | 94 kDa |
| Observed Molecular Weight | 115-120 kDa |
| GenBank Accession Number | BC007491 |
| Gene Symbol | EXO1 |
| Gene ID (NCBI) | 9156 |
| RRID | AB_2278140 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UQ84 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
EXO1, also named as HEX1, is a 846 amino acid protein, which belongs to the XPG/RAD2 endonuclease family. EXO1 subfamily. EXO1 is highly expressed in bone marrow, testis and thymus and at lower levels in colon, lymph nodes, ovary, placenta, prostate, small intestine, spleen and stomach. EXO1 as 5'->3' double-stranded DNA exonuclease, which may also possess a cryptic 3'->5' double-stranded DNA exonuclease activity. EXO1 functions in DNA mismatch repair (MMR) to excise mismatch-containing DNA tracts directed by strand breaks located either 5' or 3' to the mismatch. The calculated molecular weight of EXO1 is 94 kDa, but the modified EXO1 protein is about 115 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for EXO1/Exonuclease 1 antibody 16253-1-AP | Download protocol |
| WB protocol for EXO1/Exonuclease 1 antibody 16253-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The EXO1/Exonuclease 1 Polyclonal antibody (Cat# 16253-1-AP) has 19 citations spanning 15 journals, including Nature Cell Biology, Nature Communications, Nucleic Acids Research, Science Advances, eLife, and EMBO Molecular Medicine. Research fields covered include DNA end resection, DNA repair (mismatch repair, base excision repair), genome stability, DNA replication fork protection, antibody class switch recombination, V(D)J recombination, cancer (breast, prostate), lipid metabolism, and cardiovascular biology. Applications cited are primarily WB, with IHC and ChIP also represented.
| Species | Application | Title |
|---|---|---|
Nat Commun The RING finger E3 ligase RNF25 protects DNA replication forks independently of its canonical roles in ubiquitin signaling | ||
Nucleic Acids Res Human HELQ regulates DNA end resection at DNA double-strand breaks and stalled replication forks
| ||
Nucleic Acids Res BRD2 promotes antibody class switch recombination by facilitating DNA repair in collaboration with NIPBL | ||
Nucleic Acids Res DNA double-strand breaks with 5' adducts are efficiently channeled to the DNA2-mediated resection pathway. | ||
Reviews
What customers say
The single review for this product is highly positive (5/5). The user performed Western Blot on HeLa cells at a 1:2000 dilution and observed a single clear band at approximately 150–120 kDa. They noted the antibody performed well at this dilution and suggested it could potentially be further diluted, indicating strong sensitivity and specificity for their application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Arnaud (Verified Customer) (09-26-2025) | 1 band at 150-120 kDa (between 100-130 KDa ladder), work well at 1:2000 dilution, can be more diluted
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