EXO1/Exonuclease 1 Recombinant monoclonal antibody

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  WB analysis using 86445-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86445-1-RR (EXO1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of HEK-293 using 86445-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using EXO1 antibody (86445-1-RR, Clone: 251210D3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

EXO1/Exonuclease 1 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 86445-1-RR

Clone No.251210D3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

EXO1, EC:3.1.-.-, EXOI, Exonuclease 1, Exonuclease I

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Tested Applications

• Positive WB detected in: Raji cells, HeLa cells, HEK-293T cells, K-562 cells
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86445-1-RR targets EXO1/Exonuclease 1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag9253

  Product name: Recombinant human EXO1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 496-846 aa of BC007491

  Sequence: AVVVPGTRSRFFCSSDSTDCVSNKVSIQPLDETAVTDKENNLHESEYGDQEGKRLVDTDVARNSSDDIPNNHIPGDHIPDKATVFTDEESYSFESSKFTRTISPPTLGTLRSCFSWSGGLGDFSRTPSPSPSTALQQFRRKSDSPTSLPENNMSDVSQLKSEESSDDESHPLREGACSSQSQESGEFSLQSSNASKLSQCSSKDSDSEESDCNIKLLDSQSDQTSKLCLSHFSKKDTPLRNKVPGLYKSSSADSLSTTKIKPLGPARASGLSKKPASIQKRKHHNAENKPGLQIKLNELWKNFGFKKDSEKLPPCKKPLSPVRDNIQLTPEAEEDIFNKPECGRVQRAIFQ

• Full Name: exonuclease 1
• Calculated Molecular Weight: 94 kDa
• Observed Molecular Weight: 115-120 kDa
• GenBank Accession Number: BC007491
• Gene Symbol: EXO1
• Gene ID (NCBI): 9156
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9UQ84
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.