Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, human brain tissue, mouse brain tissue, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12014-1-AP targets Exo70 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2628 Product name: Recombinant human EXOC7 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-354 aa of BC011045 Sequence: MIPPQEASARRREIEDKLKQEEETLSFIRDSLEKSDQLTKNMVSILSSFESRLMKLENSIIPVHKQTENLQRLQENVEKTLSCLDHVISYYHVASDTEKIIREGPTGRLEEYLGSMAKIQKAVEYFQDNSPDSPELNKVKLLFERGKEALESEFRSLMTRHSKVVSPVLILDLISGDDDLEAQEDVTLEHLPESVLQDVIRISRWLVEYGRNQDFMNVYYQIRSSQLDRSIKGLKEHFHKSSSSSGVPYSPAIPNKRKDTPTKKPVKRPGTIRKAQNLLKQYSQHGLDGKKGGSNLIPLEGRDDMLDVETDAYIHCVSAFVKLAQSEYQLLADIIPEHHQKKTFDSLIQDALDG Predict reactive species |
| Full Name | exocyst complex component 7 |
| Calculated Molecular Weight | 735 aa, 83 kDa |
| Observed Molecular Weight | 74-80 kDa |
| GenBank Accession Number | BC011045 |
| Gene Symbol | Exo70 |
| Gene ID (NCBI) | 23265 |
| RRID | AB_2101698 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UPT5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Exo70 antibody 12014-1-AP | Download protocol |
| IP protocol for Exo70 antibody 12014-1-AP | Download protocol |
| WB protocol for Exo70 antibody 12014-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-Exo70 antibody (Cat# 12014-1-AP) has 14 total citations published in journals including Blood, Acta Pharmacologica Sinica, Antioxidants, Molecular Neurobiology, Biology Research, Frontiers in Pharmacology, Life Science Alliance, Placenta, Biophysical Reports, eLife, and bioRxiv. Research fields span exocyst-mediated exocytosis, neuroscience (traumatic brain injury, synapse maturation, axon formation), cancer biology, ciliogenesis, placental biology, insulin signaling in Alzheimer's disease, and blood-brain barrier function.
| Species | Application | Title |
|---|---|---|
Blood vWF maturation and release are controlled by two regulators of Weibel-Palade body biogenesis: exocyst and BLOC-2. | ||
Acta Pharmacol Sin Discovery of a novel dual-target modulator of ULK1 and ERK1/2 that suppresses triple-negative breast cancer progression and metastasis via the Exo70/Cav-1/MMPs axis. | ||
Antioxidants (Basel) Exo70 Protects Against Memory and Synaptic Impairments Following Mild Traumatic Brain Injury | ||
Mol Neurobiol The Exocyst Component Exo70 Modulates Dendrite Arbor Formation, Synapse Density, and Spine Maturation in Primary Hippocampal Neurons. | ||
Front Pharmacol ELABELA promotes the migration and homing of bone marrow mesenchymal stem cells to myocardial injury sites through the ERK1/2/miR-299a-5p/Exo70 pathway | ||
