EXOC8 Polyclonal antibody

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  WB analysis using 31523-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31523-1-AP (EXOC8 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 31523-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using EXOC8 antibody (31523-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

EXOC8 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 31523-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

SEC84, Exo84p, EXO84

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Tested Applications

• Positive WB detected in: HuH-7 cells, mouse testis tissue, rat testis tissue
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31523-1-AP targets EXOC8 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EXOC8 fusion protein Ag33300
• Full Name: exocyst complex component 8
• Calculated Molecular Weight: 725 aa, 82 kDa
• Observed Molecular Weight: 82 kDa
• GenBank Accession Number: BC035763
• Gene Symbol: EXOC8
• Gene ID (NCBI): 149371
• RRID: AB_3670020
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q8IYI6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EXOC8 is a subunit of the exocyst complex involved in docking exocytic vesicles with fusion sites on the plasma membrane. Truncated EXOC8 protein leads to the improper assembly of the exocyst complex, thus resulting in the accumulation of neurotransmitters and other excretory vesicles in the cell. It is found to play an essential role in normal cortical development and its perturbation causes complex brain disorders. EXOC8 mutations are linked to neurodevelopmental disorders with microcephaly, seizures, and brain atrophy (PMID: 35460391, PMID: 36344539).