EXOSC1 Polyclonal antibody

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  WB analysis of SGC-7901 using 12585-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 12585-1-AP (EXOSC1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis using 12585-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12585-1-AP (EXOSC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 12585-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12585-1-AP (EXOSC1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 12585-1-AP

  WB result of EXOSC1 antibody (12585-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EXOSC1 transfected MCF-7 cells.

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  WB analysis of HeLa using 12585-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12585-1-AP (EXOSC1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 12585-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12585-1-AP (EXOSC1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12585-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12585-1-AP (EXOSC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human stomach using 12585-1-AP

  human stomach tissue were subjected to SDS PAGE followed by western blot with 12585-1-AP (EXOSC1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 12585-1-AP

  IP result of anti-EXOSC1 (IP:12585-1-AP, 4ug; Detection:12585-1-AP 1:600) with HeLa cells lysate 3000 ug.

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  IHC staining of human lymphoma using 12585-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 12585-1-AP (EXOSC1 antibody) at dilution of 1:100 (under 10x lens).

• Featured Product
• KD/KO Validated

EXOSC1 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 12585-1-AP

Publications(2)

Reviews (2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IP, ELISA

Synonyms

hCsl4p, Exosome component 1, Exosome complex component CSL4, Csl4p, CSL4

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Tested Applications

• Positive WB detected in: SGC-7901 cells, HeLa cells, human stomach tissue, Jurkat cells, MCF-7 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

12585-1-AP targets EXOSC1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, yeast
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3286

  Product name: Recombinant human EXOSC1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-195 aa of BC022067

  Sequence: MAPPVRYCIPGERLCNLEEGSPGSGTYTRHGYIFSSLAGCLMKSSENGALPVVSVVRETESQLLPDVGAIVTCKVSSINSRFAKVHILYVGSMPLKNSFRGTIRKEDVRATEKDKVEIYKSFRPGDIVLAKVISLGDAQSNYLLTTAENELGVVVAHSESGIQMVPISWCEMQCPKTHTKEFRKVARVQPEFLQT

• Full Name: exosome component 1
• Calculated Molecular Weight: 195 aa, 21 kDa
• Observed Molecular Weight: 21 kDa
• GenBank Accession Number: BC022067
• Gene Symbol: EXOSC1
• Gene ID (NCBI): 51013
• RRID: AB_2101714
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9Y3B2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EXOSC1 is a core component of the exosome. The mammalian exosome is required for rapid degradation of AU rich element-containing RNAs but not for poly(A) shortening.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
yeast	WB	Am J Med Genet A; Pontocerebellar hypoplasia associated with p.Arg183Trp homozygous variant in EXOSC1 gene: A case report; Authors - Nadirah S Damseh
human	WB	Life Sci Alliance; Low expression of EXOSC2 protects against clinical COVID-19 and impedes SARS-CoV-2 replication; Authors - Tobias Moll

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tsimafei (Verified Customer) (12-17-2023)

Nice antibody!

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: mESCs

EXOSC1 Antibody Western Blot validation (1:3000 dilution) in mESCs (Cat no:12585-1-AP)

FH

Andrea (Verified Customer) (01-11-2023)

Great antibody! Were incubated overnight at 4C

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mESCs

EXOSC1 Antibody Western Blot validation (1:1000 dilution) in mESCs (Cat no:12585-1-AP)