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EXOSC1 Polyclonal antibody

EXOSC1 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 12585-1-AP

Publications (2)

Reviews (2)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IP, ELISA

Synonyms

CGI-108, CSL4, Csl4p, Exosome complex component CSL4, Exosome component 1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIPIHC

WB analysis of SGC-7901 using 12585-1-AP
SGC-7901 cells were subjected to SDS PAGE followed by western blot with 12585-1-AP (EXOSC1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis using 12585-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12585-1-AP (EXOSC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis using 12585-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12585-1-AP (EXOSC1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of MCF-7 using 12585-1-AP
WB result of EXOSC1 antibody (12585-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EXOSC1 transfected MCF-7 cells.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HeLa using 12585-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 12585-1-AP (EXOSC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HeLa using 12585-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 12585-1-AP (EXOSC1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of human stomach using 12585-1-AP
human stomach tissue were subjected to SDS PAGE followed by western blot with 12585-1-AP (EXOSC1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of Jurkat using 12585-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 12585-1-AP (EXOSC1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IP experiment of HeLa using 12585-1-AP
IP result of anti-EXOSC1 (IP:12585-1-AP, 4ug; Detection:12585-1-AP 1:600) with HeLa cells lysate 3000 ug.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human lymphoma using 12585-1-AP
Immunohistochemical analysis of paraffin-embedded human lymphoma using 12585-1-AP (EXOSC1 antibody) at dilution of 1:100 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse skin using 12585-1-AP
Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 12585-1-AP (EXOSC1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human ovary cancer using 12585-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 12585-1-AP (EXOSC1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse cerebellum using 12585-1-AP
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 12585-1-AP (EXOSC1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of rat cerebellum using 12585-1-AP
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 12585-1-AP (EXOSC1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of rat skin using 12585-1-AP
Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 12585-1-AP (EXOSC1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of rat cerebellum using 12585-1-AP
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 12585-1-AP (EXOSC1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (2)
Protocols
Reviews (2)

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	SGC-7901 cells, HeLa cells, human stomach tissue, Jurkat cells, MCF-7 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human lymphoma tissue, human ovary cancer tissue, mouse cerebellum tissue, mouse skin tissue, rat cerebellum tissue, rat skin tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12585-1-AP targets EXOSC1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, yeast
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag3286 Product name: Recombinant human EXOSC1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-195 aa of BC022067 Sequence: MAPPVRYCIPGERLCNLEEGSPGSGTYTRHGYIFSSLAGCLMKSSENGALPVVSVVRETESQLLPDVGAIVTCKVSSINSRFAKVHILYVGSMPLKNSFRGTIRKEDVRATEKDKVEIYKSFRPGDIVLAKVISLGDAQSNYLLTTAENELGVVVAHSESGIQMVPISWCEMQCPKTHTKEFRKVARVQPEFLQT Predict reactive species
Full Name	exosome component 1
Calculated Molecular Weight	195 aa, 21 kDa
Observed Molecular Weight	21 kDa
GenBank Accession Number	BC022067
Gene Symbol	EXOSC1
Gene ID (NCBI)	51013
RRID	AB_2101714
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9Y3B2
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EXOSC1 is a core component of the exosome. The mammalian exosome is required for rapid degradation of AU rich element-containing RNAs but not for poly(A) shortening.

Protocols

Product Specific Protocols
IHC protocol for EXOSC1 antibody 12585-1-AP	Download protocol
IP protocol for EXOSC1 antibody 12585-1-AP	Download protocol
WB protocol for EXOSC1 antibody 12585-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The EXOSC1 Polyclonal antibody (Cat# 12585-1-AP) has 2 citations. They appear in Life Science Alliance (2022) and American Journal of Medical Genetics Part A (2023). The cited research fields include virology/infectious disease (SARS-CoV-2 replication and clinical COVID-19) and human genetics/neurology (pontocerebellar hypoplasia linked to an EXOSC1 variant). Both citations used the antibody for Western blot applications.

Species	Application	Title
human	WB	Life Sci Alliance Low expression of EXOSC2 protects against clinical COVID-19 and impedes SARS-CoV-2 replication Authors - Tobias Moll View Article
yeast	WB	Am J Med Genet A Pontocerebellar hypoplasia associated with p.Arg183Trp homozygous variant in EXOSC1 gene: A case report Authors - Nadirah S Damseh View Article

Reviews

5.0 out of 5

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2 verified customer reviews

What customers say

Both reviewers gave this antibody a 5-star rating for Western Blot on mESCs. One used a 1:3000 dilution and described it as a "nice antibody." The other used a 1:1000 dilution with overnight incubation at 4°C, calling it a "great antibody." Both reviews were from the same lot, indicating consistent performance across users. Overall, the feedback highlights reliable WB results and ease of use in stem cell applications.

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

Tsimafei (Verified Customer) (12-17-2023)	Nice antibody! Applications: Western Blot Primary Antibody Dilution: 1:3000 Cell Tissue Type: mESCs EXOSC1 Antibody Western Blot validation (1:3000 dilution) in mESCs (Cat no:12585-1-AP)
Andrea (Verified Customer) (01-11-2023)	Great antibody! Were incubated overnight at 4C Applications: Western Blot Primary Antibody Dilution: 1:1000 Cell Tissue Type: mESCs EXOSC1 Antibody Western Blot validation (1:1000 dilution) in mESCs (Cat no:12585-1-AP)