EXOSC1 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 83875-5-RR (same clone as 83875-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83875-5-RR (EXOSC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83875-5-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 83875-5-RR (same clone as 83875-5-PBS)

  WB result of EXOSC1 antibody (83875-5-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EXOSC1 transfected MCF-7 cells. This data was developed using the same antibody clone with 83875-5-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 83875-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83875-5-RR against Human EXOSC1 were performed. The affinity constant is 1.86 nM.

• Featured Product
• KD/KO Validated

EXOSC1 Uni-rAb^® Recombinant Antibody for WB, Indirect ELISA

Cat No. 83875-5-PBS

Clone No.240938C6

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, Indirect ELISA

Synonyms

Exosome component 1, Exosome complex component CSL4, Csl4p, CSL4, CGI-108

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Product Information

83875-5-PBS targets EXOSC1 in WB, Indirect ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag3286

  Product name: Recombinant human EXOSC1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-195 aa of BC022067

  Sequence: MAPPVRYCIPGERLCNLEEGSPGSGTYTRHGYIFSSLAGCLMKSSENGALPVVSVVRETESQLLPDVGAIVTCKVSSINSRFAKVHILYVGSMPLKNSFRGTIRKEDVRATEKDKVEIYKSFRPGDIVLAKVISLGDAQSNYLLTTAENELGVVVAHSESGIQMVPISWCEMQCPKTHTKEFRKVARVQPEFLQT

• Full Name: exosome component 1
• Calculated Molecular Weight: 195 aa, 21 kDa
• Observed Molecular Weight: 21 kDa
• GenBank Accession Number: BC022067
• Gene Symbol: EXOSC1
• Gene ID (NCBI): 51013
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q9Y3B2
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

EXOSC1 is a core component of the exosome. The mammalian exosome is required for rapid degradation of AU rich element-containing RNAs but not for poly(A) shortening.