EXOSC8 Polyclonal antibody

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EXOSC8 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 11979-1-AP Copied!

Publications (12)

Reviews (3)

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Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

RRP43, Ribosomal RNA-processing protein 43, p9, Opa-interacting protein 2, OIP2

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  WB analysis of HEK-293 using 11979-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11979-1-AP (EXOSC8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 11979-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11979-1-AP (EXOSC8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human brain using 11979-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11979-1-AP (EXOSC8 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HEK-293 using 11979-1-AP

  IP result of anti-EXOSC8 (IP:11979-1-AP, 4ug; Detection:11979-1-AP 1:500) with HEK-293 cells lysate 1560ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon using 11979-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 11979-1-AP (EXOSC8 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon using 11979-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 11979-1-AP (EXOSC8 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of 4T1 using 11979-1-AP

  Immunofluorescent analysis of 4T1 cells, using EXOSC8 antibody 11979-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, human brain tissue
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: 4T1 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:800-1:3200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11979-1-AP targets EXOSC8 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2605

  Product name: Recombinant human EXOSC8 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-276 aa of BC020773

  Sequence: MAAGFKTVEPLEYYRRFLKENCRPDGRELGEFRTTTVNIGSISTADGSALVKLGNTTVICGVKAEFAAPSTDAPDKGYVVPNVDLPPLCSSRFRSGPPGEEAQVASQFIADVIENSQIIQKEDLCISPGKLVWVLYCDLICLDYDGNILDACTFALLAALKNVQLPEVTINEETALAEVNLKKKSYLNIRTHPVATSFAVFDDTLLIVDPTGEEEHLATGTLTIVMDEEGKLCCLHKPGGSGLTGAKLQDCMSRAVTRHKEVKKLMDEVIKSMKPK

• Full Name: exosome component 8
• Calculated Molecular Weight: 276 aa, 30 kDa
• Observed Molecular Weight: 30-35 kDa
• GenBank Accession Number: BC020773
• Gene Symbol: EXOSC8
• Gene ID (NCBI): 11340
• RRID: AB_2101981
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96B26
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EXOSC8 (exosome component 8), also known as p9 or CIP3, is a component of the exosome multi-enzyme ribonuclease complex, that degrades or processes messenger RNA. It belongs to the RNase PH family and localizes to the nucleolus. EXOSC8 is one of the six RNase-PH domain subunits of the exosome. EXOSC8 is part of the central hexamer channel of the exosome. EXOSC8 mutations could alter mRNA metabolism and cause hypomyelination with spinal muscular atrophy and cerebellar hypoplasia.

Publications

What published studies show

The EXOSC8 Polyclonal antibody (Cat# 11979-1-AP) has 12 citations across journals including Nature, Nature Communications, Molecular Cell, EMBO Journal, Cell Reports, JBC, Neuromuscular Disorders, Life Science Alliance, PLoS One, and Translational Cancer Research. Research fields span RNA exosome function, rRNA/mRNA processing, neurodevelopmental disorders, cancer biology (breast cancer, osteosarcoma), ribosomal subunit maturation, circular RNA degradation, and SARS-CoV-2 replication.

Automatically generated from the 12 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Nature; Nucleolar URB1 ensures 3' ETS rRNA removal to prevent exosome surveillance; Authors - Lin Shan
human	WB	Nat Commun; EXOSC8 mutations alter mRNA metabolism and cause hypomyelination with spinal muscular atrophy and cerebellar hypoplasia.; Authors - Veronika Boczonadi ; KD Validated
human	WB	Nat Commun; Loss of heterozygosity of essential genes represents a widespread class of potential cancer vulnerabilities.; Authors - Caitlin A Nichols ; KO Validated
human	WB	Mol Cell; Dual modes of ZFC3H1 confer selectivity in nuclear RNA sorting; Authors - Jing Fan
human	IF	Mol Cell; Degradation of circular RNA by the ribonuclease DIS3; Authors - Xiao Tao
		EMBO J; Cellular assembly and functional resilience of the mammalian RNA exosome.; Authors - Tsimafei Navalayeu

Reviews

5.0 out of 5

3 verified customer reviews

What customers say

All three reviews rate this antibody 5 stars for Western Blot. Users report specific Exosc8 detection in human RKO cells and mouse embryonic stem cells at dilutions of 1:2000–1:3000. One reviewer noted that the antibody still produced a satisfactory signal after two rounds of membrane stripping, indicating strong binding and durability. Overall, the feedback highlights reliable specificity, consistent performance across cell types, and robust signal intensity.

Automatically generated from the 3 verified customer reviews below by Able AI.

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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Aleksandr (Verified Customer) (12-15-2025)

Specific exosc8 detection in human cells

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: RKO

EXOSC8 Antibody Western Blot validation (1:3000 dilution) in RKO (Cat no:11979-1-AP)

Tsimafei (Verified Customer) (12-04-2022)

50µg of total protein were used. Incubation ON at 4C

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: mESCs

EXOSC8 Antibody Western Blot validation (1:2000 dilution) in mESCs (Cat no:11979-1-AP)

Nikolaus (Verified Customer) (05-24-2022)

Antibodies applied after 2 rounds of stripping, signal still satisfactory

• Applications: Western Blot
• Primary Antibody Dilution: 1/2000
• Cell Tissue Type: mESC

EXOSC8 Antibody Western Blot validation (1/2000 dilution) in mESC (Cat no:11979-1-AP)