EXOSC9 Polyclonal antibody

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  WB analysis using 24470-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24470-1-AP (EXOSC9 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 24470-1-AP

  WB result of EXOSC9 antibody (24470-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EXOSC9 transfected HeLa cells.

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  WB analysis of MCF-7 using 24470-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 24470-1-AP (EXOSC9 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate hyperplasia using 24470-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 24470-1-AP (EXOSC9 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of mouse kidney using 24470-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 24470-1-AP (EXOSC9 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 24470-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using EXOSC9 antibody (24470-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• Featured Product
• KD/KO Validated

EXOSC9 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 24470-1-AP

Publications(3)

Reviews (2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

PMSCL1, PM/Scl-75, P75 polymyositis-scleroderma overlap syndrome-associated autoantigen, Exosome complex component RRP45

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Tested Applications

• Positive WB detected in: HEK-293 cells, MCF-7 cells, HeLa cells, HepG2 cells, NIH/3T3 cells
• Positive IHC detected in: human prostate hyperplasia tissue, mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 1 publications below

Product Information

24470-1-AP targets EXOSC9 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, yeast
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag19768

  Product name: Recombinant human EXOSC9 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-351 aa of BC142978

  Sequence: MKETPLSNCERRFLLRAIEEKKRLDGRQTYDYRNIRISFGTDYGCCIVELGKTRVLGQVSCELVSPKLNRATEGILFFNLELSQMAAPAFEPGRQSDLLVKLNRLMERCLRNSKCIDTESLCVVAGEKVWQIRVDLHLLNHDGNIIDAASIAAIVALCHFRRPDVSVQGDEVTLYTPEERDPVPLSIHHMPICVSFAFFQQGAYLLVDPNEREERVMDGLLVIAMNKHREICTIQSSGGIMLLKDQVLRCSKIAGVKVAEITELILKALENDQKVRKEGGKFGFAESIANQRITAFKMEKAPIDTSDVEEKAEEIIAEAEPPSEVVSTPVLWTPGTAQIGEGVENSWGDLE

• Full Name: exosome component 9
• Calculated Molecular Weight: 456 aa, 51 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: BC142978
• Gene Symbol: EXOSC9
• Gene ID (NCBI): 5393
• RRID: AB_2879559
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q06265
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EXOSC9, also named as PMSCL1 and PM/Scl-75, is a key subunit of the exosome complex. EXOSC9 maintains self-renewal in a cell-autonomous manner. EXOSC9 has 4 isoforms with MW 49 kDa, 51 kDa, 39 kDa and 41 kDa. It's polymyositis/scleroderma auto-antigen 75 kDa. The MW of EXOSC9 is migrated to 60-75 kDa for modification in WB detection.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Life Sci Alliance; Low expression of EXOSC2 protects against clinical COVID-19 and impedes SARS-CoV-2 replication; Authors - Tobias Moll
yeast	WB	G3 (Bethesda); Humanized Saccharomyces cerevisiae provides a facile and effective tool to identify damaging human variants that cause exosomopathies; Authors - Khondakar Sayef Ahammed
human	IF	Mol Cell; Degradation of circular RNA by the ribonuclease DIS3; Authors - Xiao Tao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tsimafei (Verified Customer) (12-17-2023)

Great antibody

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: mESCs

EXOSC9 Antibody Western Blot validation (1:3000 dilution) in mESCs (Cat no:24470-1-AP)

FH

Nikolaus (Verified Customer) (07-14-2022)

Antibodies were incubated ON at 4C. Exosc9 depletion was used as a control to check the specificity of the band.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mESCs

EXOSC9 Antibody Western Blot validation (1:1000 dilution) in mESCs (Cat no:24470-1-AP)