Tested Applications
| Positive WB detected in | HEK-293 cells, MCF-7 cells, HeLa cells, HepG2 cells, NIH/3T3 cells |
| Positive IHC detected in | human prostate hyperplasia tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
24470-1-AP targets EXOSC9 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, yeast |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag19768 Product name: Recombinant human EXOSC9 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-351 aa of BC142978 Sequence: MKETPLSNCERRFLLRAIEEKKRLDGRQTYDYRNIRISFGTDYGCCIVELGKTRVLGQVSCELVSPKLNRATEGILFFNLELSQMAAPAFEPGRQSDLLVKLNRLMERCLRNSKCIDTESLCVVAGEKVWQIRVDLHLLNHDGNIIDAASIAAIVALCHFRRPDVSVQGDEVTLYTPEERDPVPLSIHHMPICVSFAFFQQGAYLLVDPNEREERVMDGLLVIAMNKHREICTIQSSGGIMLLKDQVLRCSKIAGVKVAEITELILKALENDQKVRKEGGKFGFAESIANQRITAFKMEKAPIDTSDVEEKAEEIIAEAEPPSEVVSTPVLWTPGTAQIGEGVENSWGDLE Predict reactive species |
| Full Name | exosome component 9 |
| Calculated Molecular Weight | 456 aa, 51 kDa |
| Observed Molecular Weight | 65-70 kDa |
| GenBank Accession Number | BC142978 |
| Gene Symbol | EXOSC9 |
| Gene ID (NCBI) | 5393 |
| RRID | AB_2879559 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q06265 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
EXOSC9, also named as PMSCL1 and PM/Scl-75, is a key subunit of the exosome complex. EXOSC9 maintains self-renewal in a cell-autonomous manner. EXOSC9 has 4 isoforms with MW 49 kDa, 51 kDa, 39 kDa and 41 kDa. It's polymyositis/scleroderma auto-antigen 75 kDa. The MW of EXOSC9 is migrated to 60-75 kDa for modification in WB detection.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for EXOSC9 antibody 24470-1-AP | Download protocol |
| IHC protocol for EXOSC9 antibody 24470-1-AP | Download protocol |
| WB protocol for EXOSC9 antibody 24470-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The EXOSC9 Polyclonal Antibody (Cat# 24470-1-AP) has 5 citations spanning Molecular Cell, EMBO Journal, International Journal of Molecular Sciences, Life Science Alliance, and G3 (Bethesda). Research fields include RNA exosome assembly and function, circular RNA degradation by DIS3, C9orf72 repeat transcript decay, SARS-CoV-2 replication, and exosomopathy variant identification. Cited applications include WB and IF, with reactivity in human, mouse, and yeast.
| Species | Application | Title |
|---|---|---|
Int J Mol Sci RAN Translation-Coupled Decay of the C9orf72 GGGGCC Repeat Transcript by the RNA Exosome Suppresses Dipeptide Repeat Production. | ||
Life Sci Alliance Low expression of EXOSC2 protects against clinical COVID-19 and impedes SARS-CoV-2 replication | ||
G3 (Bethesda) Humanized Saccharomyces cerevisiae provides a facile and effective tool to identify damaging human variants that cause exosomopathies |
Reviews
What customers say
All three reviews rate this antibody 5 stars for Western Blot. Users report strong specificity against human Exosc9, with one reviewer confirming detection of both protein isoforms and validating the band using Exosc9 depletion as a negative control. The antibody performed well in RKO cells and mouse embryonic stem cells at dilutions ranging from 1:1,000 to 1:5,000. Overall, reviewers describe it as a reliable, specific reagent with clear, expected bands.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Aleksandr (Verified Customer) (02-07-2026) | Specific antibody against human exosc9 that detect both protein isoforms
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Tsimafei (Verified Customer) (12-17-2023) | Great antibody
 |
Nikolaus (Verified Customer) (07-14-2022) | Antibodies were incubated ON at 4C. Exosc9 depletion was used as a control to check the specificity of the band.
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