EXOSC9 Polyclonal antibody

• Featured Product
• KD/KO Validated

EXOSC9 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Reviews (2)

Cat no : 24470-1-AP

Synonyms

Autoantigen PM/Scl 1, EXOSC9, exosome component 9, p5, p6, PM/Scl 75, PMSCL1, RRP45, Rrp45p

---

Validation Data Gallery

• 

  WB analysis using 24470-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24470-1-AP (EXOSC9 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 24470-1-AP

  WB result of EXOSC9 antibody (24470-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EXOSC9 transfected HeLa cells.

• 

  WB analysis of MCF-7 using 24470-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 24470-1-AP (EXOSC9 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate hyperplasia using 24470-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 24470-1-AP (EXOSC9 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse kidney using 24470-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 24470-1-AP (EXOSC9 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 24470-1-AP

  Immunofluorescent analysis of HeLa cells using 24470-1-AP (EXOSC9 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

---

Tested Applications

• Positive WB detected in: HEK-293 cells, MCF-7 cells, HeLa cells, HepG2 cells, NIH/3T3 cells
• Positive IHC detected in: human prostate hyperplasia tissue, mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

24470-1-AP targets EXOSC9 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EXOSC9 fusion protein Ag19768
• Full Name: exosome component 9
• Calculated Molecular Weight: 456 aa, 51 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: BC142978
• Gene Symbol: EXOSC9
• Gene ID (NCBI): 5393
• RRID: AB_2879559
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EXOSC9, also named as PMSCL1 and PM/Scl-75, is a key subunit of the exosome complex. EXOSC9 maintains self-renewal in a cell-autonomous manner. EXOSC9 has 4 isoforms with MW 49 kDa, 51 kDa, 39 kDa and 41 kDa. It's polymyositis/scleroderma auto-antigen 75 kDa. The MW of EXOSC9 is migrated to 60-75 kDa for modification in WB detection.

Publications

Species	Application	Title
human	WB	Life Sci Alliance; Low expression of EXOSC2 protects against clinical COVID-19 and impedes SARS-CoV-2 replication; Authors - Tobias Moll

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Tsimafei (Verified Customer) (12-17-2023)

Great antibody

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: mESCs

EXOSC9 Antibody Western Blot validation (1:3000 dilution) in mESCs (Cat no:24470-1-AP)

FH

Nikolaus (Verified Customer) (07-14-2022)

Antibodies were incubated ON at 4C. Exosc9 depletion was used as a control to check the specificity of the band.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mESCs

EXOSC9 Antibody Western Blot validation (1:1000 dilution) in mESCs (Cat no:24470-1-AP)