EXPH5 Polyclonal antibody

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  WB analysis of A431 using 22199-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 22199-1-AP (EXPH5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 22199-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22199-1-AP (EXPH5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 22199-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22199-1-AP (EXPH5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

EXPH5 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 22199-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

exophilin 5, EXPH5, KIAA0624, SLAC2 B

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Tested Applications

• Positive WB detected in: A431 cells
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

22199-1-AP targets EXPH5 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag17449

  Product name: Recombinant human EXPH5 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1640-1989 aa of BC113119

  Sequence: NPLFPEPTPKSAESIGESRLSENRKHVKKSENLLPITVLPNREPSTHVSNQKSNSISQRHQNEFKNVSESPSKHENSKDVTAAQNLVRESGAPSPITFTSLREAEFSDNQRRLSPPFPLEPAQKSRVSSPLASFLQQQRSASSLEWEPEPHLYRSKSLKSINVHGDLLRKSHPPKVRERHFSESTSIDNALSRLTLGNEFSVNNGYSRRFRSFSELPSCDGNESWAYRSGTKTGPRSAISIYRPIDYGIFGKEQQLAFLENVKRSLTQGRLWKPSFLKNPGFLKDDLRNPPNPSESLSSNSPSSQVPEDGLSPSEPLNIYEDDPVDSDCDTDTTTDDEYYLDENDKESEL

• Full Name: exophilin 5
• Calculated Molecular Weight: 1989 aa, 223 kDa
• Observed Molecular Weight: 222-240 kDa
• GenBank Accession Number: BC113119
• Gene Symbol: EXPH5
• Gene ID (NCBI): 23086
• RRID: AB_2879026
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• UNIPROT ID: Q8NEV8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EXPH5, also named as KIAA0624 or SLAC2B, is a 1989 amino acid protein, which contains one RabBD (Rab-binding) domain. EXPH5 is expressed in keratinocytes. EXPH5 may act as Rab effector protein and play a role in vesicle trafficking. EXPH5 plays a role in maintaining keratinocyte integrity, and in inherited skin fragility.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
		Genes Dev; CALML5 is a ZNF750- and TINCR-induced protein that binds stratifin to regulate epidermal differentiation.; Authors - Bryan K Sun