EXT2 Polyclonal antibody, PBS Only

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• EXT2 Polyclonal antibody, PBS Only (11348-1-PBS)

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  WB analysis using 11348-1-AP (same clone as 11348-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11348-1-AP (EXT2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11348-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 11348-1-AP (same clone as 11348-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 11348-1-AP (EXT2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11348-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 11348-1-AP (same clone as 11348-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11348-1-AP (EXT2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11348-1-PBS in a different storage buffer formulation.

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  IF Staining of human colon cancer using 11348-1-AP (same clone as 11348-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon cancer tissue using EXT2 antibody (11348-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11348-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human colon cancer using 11348-1-AP (same clone as 11348-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon cancer tissue using EXT2 antibody (11348-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11348-1-PBS in a different storage buffer formulation.

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  IF Staining of Jurkat using 11348-1-AP (same clone as 11348-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using EXT2 antibody (11348-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 11348-1-PBS in a different storage buffer formulation.

EXT2 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Cat No. 11348-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Synonyms

EC:2.4.1.224, Exostosin 2, EXT-2, Glucuronosyl-N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase, Heparan sulfate co-polymerase subunit EXT1

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Product Information

11348-1-PBS targets EXT2 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1748

  Product name: Recombinant human EXT2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 369-718 aa of BC010058

  Sequence: KMSDVYSILQSIPQRQIEEMQRQARWFWEAYFQSIKAIALATLQIINDRIYPYAAISYEEWNDPPAVKWGSVSNPLFLPLIPPQSQGFTAIVLTYDRVESLFRVITEVSKVPSLSKLLVVWNNQNKNPPEDSLWPKIRVPLKVVRTAENKLSNRFFPYDEIETEAVLAIDDDIIMLTSDELQFGYEVWREFPDRLVGYPGRLHLWDHEMNKWKYESEWTNEVSMVLTGAAFYHKYFNYLYTYKMPGDIKNWVDAHMNCEDIAMNFLVANVTGKAVIKVTPRKKFKCPECTAIDGLSLDQTHMVERSECINKFASVFGTMPLKVVEHRADPVLYKDDFPEKLKSFPNIGSL

• Full Name: exostoses (multiple) 2
• Calculated Molecular Weight: 718 aa, 82 kDa
• Observed Molecular Weight: 70-82 kDa
• GenBank Accession Number: BC010058
• Gene Symbol: EXT2
• Gene ID (NCBI): 2132
• RRID: AB_2293839
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q93063
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Exostosin-2 (EXT2) is type II transmembrane glycosyltransferase forming with EXT1 the heterodimeric heparan sulfate polymerase which catalyzes the elongation of the heparan sulfate glycan backbone(PMID: 22660413, 36402845, 36593275).