EYA2 Polyclonal antibody

EYA2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 11314-1-AP

Synonyms

EC:3.1.3.48, EAB1

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Validation Data Gallery

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  WB analysis using 11314-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11314-1-AP (EYA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 11314-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11314-1-AP (EYA2 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HeLa using 11314-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using EYA2 antibody (11314-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, Y79 cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

11314-1-AP targets EYA2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EYA2 fusion protein Ag1860
• Full Name: eyes absent homolog 2 (Drosophila)
• Calculated Molecular Weight: 64 kDa
• Observed Molecular Weight: 56 kDa and 70 kDa
• GenBank Accession Number: BC013882
• Gene Symbol: EYA2
• Gene ID (NCBI): 2139
• RRID: AB_10733651
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EYA2, also named EAB1, belongs to the HAD-like hydrolase superfamily and EYA family. It coactivates SIX1. The Six1-EYA2 interaction may inhibit breast cancer progression. EYA2 is required for Six1-activated TGF-beta signaling.(PMID:21706047) It may be involved in the development of the eye. This protein has 3 isoforms produced by alternative splicing with the molecular weight of 59 kDa, 57 kDa, and 50 kDa. It can be detected two bands of 70 kDa and 56 kDa in the neuroblastoma cells by western blot(PMID:12039049).

Publications

Species	Application	Title
human	IHC,IF	Oncogene; Eya2 is required to mediate the pro-metastatic functions of Six1 via the induction of TGF-β signaling, epithelial-mesenchymal transition, and cancer stem cell properties.; Authors - Farabaugh S M SM
mouse	WB	Front Cell Dev Biol; A Phosphotyrosine Switch in Estrogen Receptor β Is Required for Mouse Ovarian Function.; Authors - Bin Yuan
human	IHC,WB	Int J Mol Med; Eya2 overexpression promotes the invasion of human astrocytoma through the regulation of ERK/MMP9 signaling.; Authors - Zhifeng Wen