Validation Data Gallery
|Positive WB detected in||HeLa cells, L02 cells|
|Positive IP detected in||L02 cells|
|Positive IHC detected in||human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||Hela cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
The immunogen of 21196-1-AP is EYA3 Fusion Protein expressed in E. coli.
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||EYA3 fusion protein Ag15544|
|Full Name||eyes absent homolog 3 (Drosophila)|
|Calculated molecular weight||573 aa, 63 kDa|
|Observed molecular weight||63-66 kDa|
|GenBank accession number||BC029500|
|Gene ID (NCBI)||2140|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
EYA3(Eyes absent homolog 3) belongs to the HAD-like hydrolase superfamily.Its function as histone phosphatase probably explains its role in transcription regulation during organogenesis.EYA3, like EYA1, is expressed in the ninth week of human development and may also underlie developmental defects(PMID:9020840).It has 2 isoforms produced by alternative splicing.This antibody is specific to EYA3.
|Product Specific Protocols|
|WB protocol for EYA3 antibody 21196-1-AP||Download protocol|
|IHC protocol for EYA3 antibody 21196-1-AP||Download protocol|
|IF protocol for EYA3 antibody 21196-1-AP||Download protocol|
|IP protocol for EYA3 antibody 21196-1-AP||Download protocol|
|Click here to view our Standard Protocols|
The EYA3 tyrosine phosphatase activity promotes pulmonary vascular remodeling in pulmonary arterial hypertension.
WDR1 is a novel EYA3 substrate and its dephosphorylation induces modifications of the cellular actin cytoskeleton.
Int J Mol Sci
Analysis of EYA3 Phosphorylation by Src Kinase Identifies Residues Involved in Cell Proliferation.
Structure-Activity Relationships of Benzbromarone Metabolites and Derivatives as EYA Inhibitory Anti-Angiogenic Agents.
Ann Transl Med
Both a hypoxia-inducible EYA3 and a histone acetyltransferase p300 function as coactivators of SIX5 to mediate tumorigenesis and cancer progression