EYA3 Polyclonal antibody

EYA3 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 21196-1-AP

Synonyms

EYA3, Eyes absent homolog 3



Tested Applications

Positive WB detected inHeLa cells, L02 cells
Positive IP detected inL02 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 21196-1-AP is EYA3 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EYA3 fusion protein Ag15544
Full Name eyes absent homolog 3 (Drosophila)
Calculated molecular weight 573 aa, 63 kDa
Observed molecular weight 63-66 kDa
GenBank accession numberBC029500
Gene symbol EYA3
Gene ID (NCBI) 2140
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

EYA3(Eyes absent homolog 3) belongs to the HAD-like hydrolase superfamily.Its function as histone phosphatase probably explains its role in transcription regulation during organogenesis.EYA3, like EYA1, is expressed in the ninth week of human development and may also underlie developmental defects(PMID:9020840).It has 2 isoforms produced by alternative splicing.This antibody is specific to EYA3.

Protocols

Product Specific Protocols
WB protocol for EYA3 antibody 21196-1-APDownload protocol
IHC protocol for EYA3 antibody 21196-1-APDownload protocol
IF protocol for EYA3 antibody 21196-1-APDownload protocol
IP protocol for EYA3 antibody 21196-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

PLoS One

Structure-Activity Relationships of Benzbromarone Metabolites and Derivatives as EYA Inhibitory Anti-Angiogenic Agents.

Authors - Ram Naresh Pandey
humanWB,IP

Int J Mol Sci

Analysis of EYA3 Phosphorylation by Src Kinase Identifies Residues Involved in Cell Proliferation.

Authors - Aura E Ionescu

Sci Rep

WDR1 is a novel EYA3 substrate and its dephosphorylation induces modifications of the cellular actin cytoskeleton.

Authors - Mihaela Mentel
humanWB,IHC,IF

Nat Commun

The EYA3 tyrosine phosphatase activity promotes pulmonary vascular remodeling in pulmonary arterial hypertension.

Authors - Yuhua Wang