Enpep Polyclonal antibody

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• Enpep Polyclonal antibody (33251-1-AP)

• 

  WB analysis using 33251-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 33251-1-AP (Enpep antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Enpep Polyclonal Antibody for WB, ELISA

Cat No. 33251-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, ELISA

Synonyms

Aminopeptidase A, AP-A, BP-1/6C3 antigen, CD249, EAP

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Tested Applications

• Positive WB detected in: mouse lung tissue, rat lung tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

33251-1-AP targets Enpep in WB, ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Eg4233

  Product name: Recombinant Mouse ENPEP protein (rFc Tag)

  Source: mammalian cells-derived, pHZ-KIsec-N-rFc

  Tag: N-rFc

  Domain: 41-945 aa of NM_007934.3

  Sequence: RSCEQDTTPAPSQPPPEASTALPPQDQNVCPDSEDESGEWKNFRLPDFINPVHYDLEVKALMEEDRYTGIVTISVNLSKPTRDLWLHIRETKITKLPELRRPSGEQVPIRRCFEYKKQEYVVIQAAEDLAATSGDSVYRLTMEFKGWLNGSLVGFYKTTYMEDGQIRSIAATDHEPTDARKSFPCFDEPNKKSTYSISIIHPKEYSALSNMPEEKSEMVDDNWKKTTFVKSVPMSTYLVCFAVHRFTAIERKSRSGKPLKVYVQPNQKETAEYAANITQAVFDYFEDYFAMEYALPKLDKIAIPDFGTGAMENWGLVTYRETNLLYDPLLSASSNQQRVASVVAHELVHQWFGNTVTMDWWDDLWLNEGFASFFEFLGVNHAEKDWQMLSQVLLEDVFPVQEDDSLMSSHPVVVTVSTPAEITSVFDGISYSKGASILRMLQDWITPEKFQKGCQIYLKKFQFANAKTSDFWDSLQEASNLPVKEVMDTWTSQMGYPVVTVSGRQNITQKRFLLDSKADPSQPPSELGYTWNIPVRWADNDNSRITVYNRLDKGGITLNANLSGDAFLKINPDHIGFYRVNYEGGTWDWIAEALSSNHTRFSAADRSSFIDDAFALARAQLLNYKIALNLTMYLKSEEDFLPWERVISSVSYIISMFEDDRELYPMIETYFQGQVKPVADLLGWQDTGSHITKLLRASILGFACKMGDREALGNASQLFDSWLKGSASIPVNLRLLVYRYGMQNSGNEAAWNYTLEQYQKTSLAQEKEKLLYGLASVKDVKLLARYLEMLKDPNIIKTQDVFTVIRYISYNSYGKTMAWNWIQLNWDYLVSRFTINDRYLGRIVTIAEPFNTELQLWQMQSFFAKYPNAGAGAKPREQVLETVKNNIEWLNVNRQSIREWFASLP

• Full Name: glutamyl aminopeptidase
• Calculated Molecular Weight: 108 kDa
• Observed Molecular Weight: 150 kDa
• GenBank Accession Number: NM_007934.3
• Gene Symbol: Enpep
• Gene ID (NCBI): 13809
• RRID: AB_3742914
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: P16406
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The gene ENPEP encodes glutamyl aminopeptidase, which can cut N‐terminal aspartic acid from angiotensin II, and is related to tumorigenesis and immune microenvironment. ENPEP is a potential biomarker to classify patients' response to ICIs treatment (PMID: 34862755). This protein has multiple glycosylation sites: 4 N-linked glycans (4 sites), 1 O-linked glycan (1 site).