Entactin Monoclonal antibody

Entactin Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Pig, Mouse, Rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2A11G1

Cat no : 67310-1-Ig

Synonyms

Entactin, NID, NID 1, NID1, nidogen 1

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Validation Data Gallery

• 

  WB analysis of mouse kidney using 67310-1-Ig

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 67310-1-Ig (Entactin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 67310-1-Ig

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 67310-1-Ig (Entactin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 67310-1-Ig

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 67310-1-Ig (Entactin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 67310-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67310-1-Ig (Entactin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 67310-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67310-1-Ig (Entactin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig heart using 67310-1-Ig

  pig heart tissue were subjected to SDS PAGE followed by western blot with 67310-1-Ig (Entactin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 67310-1-Ig

  rat heart tissue were subjected to SDS PAGE followed by western blot with 67310-1-Ig (Entactin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary tumor using 67310-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67310-1-Ig (Entactin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 67310-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67310-1-Ig (Entactin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse kidney tissue, rat kidney tissue, HeLa cells, human placenta tissue, pig heart tissue, rat heart tissue, mouse heart tissue
• Positive IHC detected in: human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67310-1-Ig targets Entactin in WB, IHC, ELISA applications and shows reactivity with Human, Pig, Mouse, Rat samples.

• Tested Reactivity: Human, Pig, Mouse, Rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Entactin fusion protein Ag4739
• Full Name: nidogen 1
• Calculated Molecular Weight: 1247 aa, 136 kDa
• Observed Molecular Weight: 150 kDa
• GenBank Accession Number: BC045606
• Gene Symbol: NID1
• Gene ID (NCBI): 4811
• RRID: AB_2882570
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.