Tested Applications
| Positive WB detected in | mouse kidney tissue, rat kidney tissue, HeLa cells, human placenta tissue, pig heart tissue, rat heart tissue, mouse heart tissue |
| Positive IHC detected in | human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67310-1-Ig targets Entactin in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with Human, Pig, Mouse, Rat samples.
| Tested Reactivity | Human, Pig, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4739 Product name: Recombinant human NID1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 28-400 aa of BC045606 Sequence: CLSRQELFPFGPGQGDLELEDGDDFVSPALELRGALRFYDRSDIDAVYVTTNGIIATSEPPAKESHPGLFPPTFGAVAPFLADLDTTDGLGKVYYREDLSPSITQRAAECVHRGFPEISFQPSSAVVVTWESVAPYQGPSRDPDQKGKRNTFQAVLASSDSSSYAIFLYPEDGLQFHTTFSKKENNQVPAVVAFSQGSVGFLWKSNGAYNIFANDRESIENLAKSSNSGQQGVWVFEIGSPATTNGVVPADVILGTEDGAEYDDEDEDYDLATTRLGLEDVGTTPFSYKALRRGGADTYSVPSVLSPRRAATERPLGPPTERTRSFQLAVETFHQQHPQVIDVDEVEETGVVFSYNTDSRQTCANNRHQCSVH Predict reactive species |
| Full Name | nidogen 1 |
| Calculated Molecular Weight | 1247 aa, 136 kDa |
| Observed Molecular Weight | 150 kDa |
| GenBank Accession Number | BC045606 |
| Gene Symbol | Entactin/NID |
| Gene ID (NCBI) | 4811 |
| RRID | AB_2882570 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P14543 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Entactin antibody 67310-1-Ig | Download protocol |
| IHC protocol for Entactin antibody 67310-1-Ig | Download protocol |
| WB protocol for Entactin antibody 67310-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Entactin Monoclonal antibody (2A11G1), catalog number 67310-1-Ig, has 1 total citation. It appears in the International Journal of Molecular Sciences (Impact Factor 5.6), published in March 2025. The cited study, "Nidogen-1, a Player in KMT2A-Rearranged Pediatric Acute Myeloid Leukemia," by Jolien Vanhooren et al., places this antibody in the research field of pediatric oncology, specifically investigating the role of Nidogen-1 in KMT2A-rearranged acute myeloid leukemia.
| Species | Application | Title |
|---|---|---|
Reviews
What customers say
Both reviewers compared this antibody against multiple other NID1 antibodies and found it to be the best performer. One user (3/5) used it at 1:1000 for IHC and IF on NHP placenta, noting specific staining consistent with published literature. The other (5/5) used it at 1:1000 for IHC on placenta, describing it as the cleanest and best-looking result among the antibodies tested. Overall, users report superior specificity and signal quality relative to alternatives they evaluated.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Avelina (Verified Customer) (04-02-2025) | We titrated 5 different NID1 antibodies and this one performed the best. Current staining appears specific and relevant to what we find in literature.
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Jillian (Verified Customer) (03-27-2025) | We tested multiple NID1 antibodies and this was the cleanest and best looking one that we recieved
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