Entactin Monoclonal antibody

Entactin Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Pig, Mouse, Rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2A11G1

Cat no : 67310-1-Ig

Synonyms

Entactin, NID, NID 1, NID1, nidogen 1



Tested Applications

Positive WB detected inmouse kidney tissue, rat kidney tissue, HeLa cells, human placenta tissue, pig heart tissue, rat heart tissue, mouse heart tissue
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67310-1-Ig targets Entactin in WB, IHC, ELISA applications and shows reactivity with Human, Pig, Mouse, Rat samples.

Tested Reactivity Human, Pig, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Entactin fusion protein Ag4739
Full Name nidogen 1
Calculated Molecular Weight 1247 aa, 136 kDa
Observed Molecular Weight 150 kDa
GenBank Accession NumberBC045606
Gene Symbol Entactin/NID
Gene ID (NCBI) 4811
RRIDAB_2882570
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for Entactin antibody 67310-1-IgDownload protocol
IHC protocol for Entactin antibody 67310-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols