Tested Applications
| Positive WB detected in | Caki-1 cells, SKOV-3 cells, U-87 MG cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
55080-1-AP targets Ephrin B3 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | ephrin-B3 |
| Calculated Molecular Weight | 36 kDa |
| Observed Molecular Weight | 45-50 kDa |
| GenBank Accession Number | NM_001406 |
| Gene Symbol | EFNB3 |
| Gene ID (NCBI) | 1949 |
| RRID | AB_3743199 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q15768 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Ephrin-B3 is a transmembrane ligand that binds primarily to EphB receptors to mediate bidirectional cell signaling, crucial for neural development, axon guidance, and synaptic plasticity. Highly expressed in the brain and spinal cord, it acts as a midline repellent to guide axonal pathfinding (PMID: 16951161). Ephrin‑B3 is important in the regulation of cell proliferation, differentiation and migration via cell‑cell contact, and can activate the reelin pathway during brain development. The band of ephrin-B3 was identical to phosphorylated ephrin-B at 50 kDa (PMID: 16951161, 21447033).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Ephrin B3 antibody 55080-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
