Estradiol Monoclonal antibody

Estradiol Monoclonal Antibody for ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Estradiol

Applications

ELISA

Conjugate

Unconjugated

CloneNo.

2E5E4

Cat no : 68600-1-Ig

Synonyms

17 beta oestradiol, beta Estradiol, C18H24O2, cis-Estradiol, Estradiol, Estradiol hemihydrate, Estradiolum

---

Validation Data Gallery

• 

  ELISA experiment of Estradiol using 68600-1-Ig

  Indirect ELISA was performed by coating BSA-Estradiol ~30 ng/well (by Estradiol amount), followed by blocking with 1% BSA. Serial diluted Estradiol antibody 68600-1-Ig was added to the plates and incubated at 37℃. HRP-Goat anti-mouse was used for detection (top X-right Y, blue curve). Competitive ELISA was performed similarly except that different concentration of Estradiol was mixed in 30ng/mL primary antibody (bottom X-left Y, red curve).

• 

  ELISA experiment of Estradiol using 68600-1-Ig

  BSA-Estradiol was coated in 96 well plate at ~30 ng/well (by Estradiol amount), followed by blocking with 1% BSA. Different concentrations of Estradiol standard as well as diluted urine from different donor were mixed with 30 ng/mL Estradiol antibody 68600-1-Ig respectively. Urine Estradiol was calculated based on standard curve. Please notice that the level from different donor might be different between individuals.

---

Tested Applications

• Positive ELISA detected in: Estradiol

Recommended dilution

• Enzyme-linked Immunosorbent Assay (ELISA): ELISA : 1:5000-1:20000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68600-1-Ig targets Estradiol in ELISA applications and shows reactivity with Estradiol samples.

• Tested Reactivity: Estradiol
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PTG
• Full Name: Estradiol
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Estradiol is a naturally occurring hormone circulating endogenously in females. It is commercially available in several hormone therapy products for managing conditions associated with reduced estrogen, such as vulvovaginal atrophy and hot flashes. Some available forms of estradiol include oral tablets, injections, vaginal rings, transdermal patches, sprays, gels, and creams. This antibody can be used in the Elisa experiments.