Estradiol Monoclonal antibody

Estradiol Monoclonal Antibody for ELISA

Cat No. 68600-1-Ig

Clone No. 2E5E4

Publications (1)

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Host / Isotype

Mouse / IgG1

Reactivity

Estradiol and More (1)

Applications

ELISA

Synonyms

17 beta oestradiol, beta Estradiol, C18H24O2, cis-Estradiol, Estradiol, Estradiol hemihydrate, Estradiolum

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

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ELISA experiment of Estradiol using 68600-1-Ig
Indirect ELISA was performed by coating BSA-Estradiol ~30 ng/well (by Estradiol amount), followed by blocking with 1% BSA. Serial diluted Estradiol antibody 68600-1-Ig was added to the plates and incubated at 37℃. HRP-Goat anti-mouse was used for detection (top X-right Y, blue curve). Competitive ELISA was performed similarly except that different concentration of Estradiol was mixed in 30ng/mL primary antibody (bottom X-left Y, red curve).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
Indirect ELISA was performed by coating BSA-Estradiol ~30 ng/well (by Estradiol amount), followed by blocking with 1% BSA. Serial diluted Estradiol antibody 68600-1-Ig was added to the plates and incubated at 37℃. HRP-Goat anti-mouse was used for detection (top X-right Y, blue curve). Competitive ELISA was performed similarly except that different concentration of Estradiol was mixed in 30ng/mL primary antibody (bottom X-left Y, red curve).
ELISA experiment of Estradiol using 68600-1-Ig
BSA-Estradiol was coated in 96 well plate at ~30 ng/well (by Estradiol amount), followed by blocking with 1% BSA. Different concentrations of Estradiol standard as well as diluted urine from different donor were mixed with 30 ng/mL Estradiol antibody 68600-1-Ig respectively. Urine Estradiol was calculated based on standard curve. Please notice that the level from different donor might be different between individuals.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
BSA-Estradiol was coated in 96 well plate at ~30 ng/well (by Estradiol amount), followed by blocking with 1% BSA. Different concentrations of Estradiol standard as well as diluted urine from different donor were mixed with 30 ng/mL Estradiol antibody 68600-1-Ig respectively. Urine Estradiol was calculated based on standard curve. Please notice that the level from different donor might be different between individuals.

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Overview

Tested Applications
Product Information
Publications (1)

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MSDS SDS

Tested Applications

Positive ELISA detected in

Estradiol

Recommended dilution

Application	Dilution
Enzyme-linked Immunosorbent Assay (ELISA)	ELISA : 1:5000-1:20000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68600-1-Ig targets Estradiol in ELISA applications and shows reactivity with Estradiol samples.

Tested Reactivity	Estradiol
Cited Reactivity	mouse
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	PTG Predict reactive species
Full Name	Estradiol
Gene Symbol
Gene ID (NCBI)
RRID	AB_3085297
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Estradiol is a naturally occurring hormone circulating endogenously in females. It is commercially available in several hormone therapy products for managing conditions associated with reduced estrogen, such as vulvovaginal atrophy and hot flashes. Some available forms of estradiol include oral tablets, injections, vaginal rings, transdermal patches, sprays, gels, and creams. This antibody can be used in the Elisa experiments.

Publications

What published studies show

The Estradiol Monoclonal antibody (2E5E4), catalog number 68600-1-Ig, has 1 total citation. The citation appears in Molecules (Impact Factor 5.1), published in 2026. The cited study focuses on the synthesis and biological evaluation of isomeric artemisinin trimers as novel antitumor agents, placing it in the research fields of medicinal chemistry and anticancer drug discovery. The antibody was used with mouse species in this publication.