Exportin 4 Recombinant antibody, PBS Only

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  WB analysis using 84449-1-RR (same clone as 84449-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84449-1-RR (XPO4 antibody) at dilution of 1:8500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84449-1-PBS in a different storage buffer formulation.

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  IF Staining of K-562 using 84449-1-RR (same clone as 84449-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed K-562 cells using Exportin 4 antibody (84449-1-RR, Clone: 241443A5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84449-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 84449-1-RR (same clone as 84449-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Exportin 4 Recombinant antibody (84449-1-RR, Clone:241443A5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84449-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84449-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84449-1-RR against Human Exportin 4 were performed. The affinity constant is 0.512 nM.

Exportin 4 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Cat No. 84449-1-PBS

Clone No.241443A5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

XPO4, 241443A5, Exp4, Exportin-4, KIAA1721

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Product Information

84449-1-PBS targets Exportin 4 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Exportin 4 fusion protein Ag21845
• Full Name: exportin 4
• Calculated Molecular Weight: 1151 aa, 130 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: BC113571
• Gene Symbol: Exportin 4/XPO4
• Gene ID (NCBI): 64328
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q9C0E2
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

exportin 4 (XPO4) maps to chromosome 13q and belongs to the importin β family which acts as a bidirectional nuclear transport receptor. Decreased XPO4 expression has been shown to associate with hepatocellular carcinoma. A genome-wide copy number variation (CNV) association study in a small cohort of Malaysian subjects identified a CNV in XPO4 to be correlated with the progression of metabolic (dysfunction) associated fatty liver disease (MAFLD). (PMID: 34388518)