F2 Polyclonal antibody

F2 Polyclonal Antibody for WB, IP, IF, ELISA

Host / Isotype

Rabbit / IgG


human and More (1)





Cat no : 24295-1-AP


Coagulation factor II, F2, Prothrombin, PT

Tested Applications

Positive WB detected inhuman plasma tissue
Positive IP detected inhuman plasma tissue
Positive IF detected inHUVEC cells

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24295-1-AP targets F2 in WB, IP, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, Plasmodium falciparum
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen F2 fusion protein Ag18385
Full Name coagulation factor II (prothrombin)
Calculated Molecular Weight 622 aa, 70 kDa
Observed Molecular Weight 72 kDa
GenBank Accession NumberBC051332
Gene Symbol F2
Gene ID (NCBI) 2147
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Normal blood coagulation is a complex process, involving a cascade of activation of different plasma proteins, ultimately resulting in the formation of a clot, called fibrin. Factor II, or prothrombin, is one of the components of this chain of plasma proteins involved in blood coagulation. Thrombin is essential in the processes of hemostasis and thrombosis.


Product Specific Protocols
WB protocol for F2 antibody 24295-1-APDownload protocol
IF protocol for F2 antibody 24295-1-APDownload protocol
IP protocol for F2 antibody 24295-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Elke (Verified Customer) (02-23-2023)

it can work on the western blot

  • Applications: Western Blot