F2RL3 Polyclonal antibody

F2RL3 Polyclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, FC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 25306-1-AP

Synonyms

F2RL3, PAR 4, PAR4, Proteinase activated receptor 4, Thrombin receptor like 3

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Validation Data Gallery

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  WB analysis using 25306-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25306-1-AP (F2RL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 25306-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 25306-1-AP (F2RL3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse pancreas using 25306-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 25306-1-AP (F2RL3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse lung using 25306-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 25306-1-AP (F2RL3 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of mouse lung using 25306-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 25306-1-AP (F2RL3 Antibody) at dilution of 1:50 (under 40x lens).

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  FC experiment of HeLa using 25306-1-AP

  1X10^6 HeLa cells were stained with 0.2ug F2RL3 antibody (25306-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Tested Applications

• Positive WB detected in: mouse lung tissue, mouse pancreas tissue
• Positive IHC detected in: mouse lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 3 publications below

Product Information

The immunogen of 25306-1-AP is F2RL3 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: F2RL3 fusion protein Ag20801
• Full Name: coagulation factor II (thrombin) receptor-like 3
• Calculated molecular weight: 385 aa, 41 kDa
• Observed molecular weight: 41 kDa
• GenBank accession number: BC074782
• Gene symbol: F2RL3
• Gene ID (NCBI): 9002
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Coagulation factor II receptor-like 3 (F2RL3) encodes a member of the protease-activated receptor subfamily, also known as protease-activated receptor 4 (PAR4), which takes partin platelet activation, intimal hyperplasia and inflammation (PMID:34284820). An absence of PAR4 in mouse models results in impaired hemostasis and a protection against pulmonary embolism, and a small number of missense coding variants in F2RL3 that alter platelet aggregation and function have been described(PMID:35012325). The calculated molecular weight and observed molecular weight of F2RL3 are both 41 kDa.

Publications

Species	Application	Title
human	WB	Oxid Med Cell Longev; Combination of Colchicine and Ticagrelor Inhibits Carrageenan-Induced Thrombi in Mice.; Authors - BuChun Zhang
mouse	WB	Front Oncol; Tumor Suppressor Par-4 Regulates Complement Factor C3 and Obesity.; Authors - Nathalia Araujo
mouse	WB	Neurosci Bull; Antagonism of Protease-Activated Receptor 4 Protects Against Traumatic Brain Injury by Suppressing Neuroinflammation via Inhibition of Tab2/NF-κB Signaling.; Authors - Jianing Luo

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