F2RL3 Polyclonal antibody

F2RL3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse





Cat no : 25306-1-AP


F2RL3, PAR 4, PAR4, Proteinase activated receptor 4, Thrombin receptor like 3

Tested Applications

Positive WB detected inmouse lung tissue, mouse pancreas tissue
Positive IHC detected inmouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

The immunogen of 25306-1-AP is F2RL3 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen F2RL3 fusion protein Ag20801
Full Name coagulation factor II (thrombin) receptor-like 3
Calculated Molecular Weight 385 aa, 41 kDa
Observed Molecular Weight 41 kDa
GenBank Accession NumberBC074782
Gene Symbol F2RL3
Gene ID (NCBI) 9002
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Coagulation factor II receptor-like 3 (F2RL3) encodes a member of the protease-activated receptor subfamily, also known as protease-activated receptor 4 (PAR4), which takes partin platelet activation, intimal hyperplasia and inflammation (PMID:34284820). An absence of PAR4 in mouse models results in impaired hemostasis and a protection against pulmonary embolism, and a small number of missense coding variants in F2RL3 that alter platelet aggregation and function have been described(PMID:35012325). The calculated molecular weight and observed molecular weight of F2RL3 are both 41 kDa.


Product Specific Protocols
WB protocol for F2RL3 antibody 25306-1-APDownload protocol
IHC protocol for F2RL3 antibody 25306-1-APDownload protocol
FC protocol for F2RL3 antibody 25306-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Oxid Med Cell Longev

Combination of Colchicine and Ticagrelor Inhibits Carrageenan-Induced Thrombi in Mice.

Authors - BuChun Zhang

Front Oncol

Tumor Suppressor Par-4 Regulates Complement Factor C3 and Obesity.

Authors - Nathalia Araujo

Neurosci Bull

Antagonism of Protease-Activated Receptor 4 Protects Against Traumatic Brain Injury by Suppressing Neuroinflammation via Inhibition of Tab2/NF-κB Signaling.

Authors - Jianing Luo