F2RL3 Polyclonal antibody

F2RL3 Polyclonal Antibody for FC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, FC,ELISA

Conjugate

Unconjugated

Cat no : 25306-1-AP

Synonyms

F2RL3, PAR 4, PAR4, Proteinase activated receptor 4, Thrombin receptor like 3



Tested Applications

Positive WB detected inmouse lung tissue, mouse pancreas tissue
Positive IHC detected inmouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 25306-1-AP is F2RL3 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen F2RL3 fusion protein Ag20801
Full Name coagulation factor II (thrombin) receptor-like 3
Calculated molecular weight 385 aa, 41 kDa
Observed molecular weight 41 kDa
GenBank accession numberBC074782
Gene symbol F2RL3
Gene ID (NCBI) 9002
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for F2RL3 antibody 25306-1-APDownload protocol
IHC protocol for F2RL3 antibody 25306-1-APDownload protocol
FC protocol for F2RL3 antibody 25306-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Neurosci Bull

Antagonism of Protease-Activated Receptor 4 Protects Against Traumatic Brain Injury by Suppressing Neuroinflammation via Inhibition of Tab2/NF-κB Signaling.

Authors - Jianing Luo