Tested Applications
| Positive WB detected in | mouse lung tissue, mouse pancreas tissue |
| Positive IHC detected in | mouse lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25306-1-AP targets F2RL3 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag20801 Product name: Recombinant human F2RL3 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 305-385 aa of BC074782 Sequence: LHYSDPSPSAWGNLYGAYVPSLALSTLNSCVDPFIYYYVSAEFRDKVRAGLFQRSPGDTVASKASAEGGSRGMGTHSSLLQ Predict reactive species |
| Full Name | coagulation factor II (thrombin) receptor-like 3 |
| Calculated Molecular Weight | 385 aa, 41 kDa |
| Observed Molecular Weight | 45 kDa |
| GenBank Accession Number | BC074782 |
| Gene Symbol | F2RL3 |
| Gene ID (NCBI) | 9002 |
| RRID | AB_2880022 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96RI0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Coagulation factor II receptor-like 3 (F2RL3) encodes a member of the protease-activated receptor subfamily, also known as protease-activated receptor 4 (PAR4), which takes partin platelet activation, intimal hyperplasia and inflammation (PMID:34284820). An absence of PAR4 in mouse models results in impaired hemostasis and a protection against pulmonary embolism, and a small number of missense coding variants in F2RL3 that alter platelet aggregation and function have been described(PMID:35012325). The calculated molecular weight and observed molecular weight of F2RL3 are both 41 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for F2RL3 antibody 25306-1-AP | Download protocol |
| WB protocol for F2RL3 antibody 25306-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The F2RL3 Polyclonal antibody (Cat# 25306-1-AP) has 6 total citations. These appear in Oxid Med Cell Longev, Neurosci Bull, Cells, Front Biosci (Landmark Ed), Front Oncol, and bioRxiv. Research fields span thrombosis and platelet activation, neuroinflammation in traumatic brain injury, adipogenesis and obesity, epithelial-mesenchymal transition in gastric cancer, and complement regulation. Applications cited include WB, IHC, and IP in human and mouse samples.
| Species | Application | Title |
|---|---|---|
Oxid Med Cell Longev Combination of Colchicine and Ticagrelor Inhibits Carrageenan-Induced Thrombi in Mice. | ||
Neurosci Bull Antagonism of Protease-Activated Receptor 4 Protects Against Traumatic Brain Injury by Suppressing Neuroinflammation via Inhibition of Tab2/NF-κB Signaling. | ||
Cells The Tumor Suppressor Par-4 Regulates Adipogenesis by Transcriptional Repression of PPARγ | ||
Front Biosci (Landmark Ed) F2RL3 Regulates Epithelial-Mesenchymal Transition and Angiogenesis in Gastric Cancer through the Rap1/MAPK Signaling Pathway. | ||
bioRxiv Sodium/Potassium ATPase Alpha 1 Subunit Fine-tunes Platelet GPCR Signaling Function and is Essential for Thrombosis. |
