Factor V Polyclonal antibody, PBS Only

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• Factor V Polyclonal antibody, PBS Only (20963-1-PBS)

• 

  WB analysis of mouse liver using 20963-1-AP (same clone as 20963-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 20963-1-AP (F5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20963-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse liver using 20963-1-AP (same clone as 20963-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 20963-1-AP (F5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20963-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 20963-1-AP (same clone as 20963-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20963-1-AP (F5 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20963-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 20963-1-AP (same clone as 20963-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20963-1-AP (F5 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20963-1-PBS in a different storage buffer formulation.

Factor V Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 20963-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

F5, Coagulation factor V heavy chain, Coagulation factor V light chain, FVL, PCCF

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Product Information

20963-1-PBS targets Factor V in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: coagulation factor V (proaccelerin, labile factor)
• Calculated Molecular Weight: 252 kDa
• Observed Molecular Weight: 145 kDa
• GenBank Accession Number: NM_000130
• Gene Symbol: F5
• Gene ID (NCBI): 2153
• RRID: AB_2878773
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P12259
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

There are 13 blood coagulation factors found in the blood. There are 13 blood coagulation factors found in the blood. F5 has little or no intrinsic procoagulant activity prior to its activation through limited proteolysis by thrombin or F10a at Arg709, Arg1018 and Arg1545. The resulting F5a molecule is a heterodimer that consists of a 105 kDa heavy chain and a 71/74 kDa light chain that are noncovalently associated in a calcium-dependent manner. The intact procofactor molecule (330 kDa) as well as proteins similar in size to the factor Va light chain (74 kDa) and both light chain precursors (280 and 220 kDa) found in factor Va(IIa) were observed. And factor Xa cleaves the factor Va light chain at Arg1765 into fragments of ~30 and ~48 kDa (residues 1546-1765 and 1766-2196, respectively) in the bovine and human systems.