FABP1 Monoclonal antibody

FABP1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1B6C8

Cat no : 68227-1-Ig

Synonyms

FABP1, FABPL, Fatty acid binding protein 1, L FABP

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Validation Data Gallery

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  WB analysis using 68227-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68227-1-Ig (FABP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig liver using 68227-1-Ig

  pig liver tissue were subjected to SDS PAGE followed by western blot with 68227-1-Ig (FABP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: rat liver tissue, pig liver tissue, mouse liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68227-1-Ig targets FABP1 in WB, ELISA applications and shows reactivity with Human, pig, mouse, rat samples.

• Tested Reactivity: Human, pig, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: FABP1 fusion protein Ag32836
• Full Name: fatty acid binding protein 1, liver
• Calculated Molecular Weight: 127 aa, 14 kDa
• Observed Molecular Weight: 14 kDa
• GenBank Accession Number: BC032801
• Gene Symbol: FABP1
• Gene ID (NCBI): 2168
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FABP1, liver fatty acid-binding protein, is abundant in cytoplasm that regulates lipid transport and metabolism. It plays a role in lipoprotein-mediated cholesterol uptake in hepatocytes (PMID:25732850). FABP1 is mainly expressed in hepatocytes, enterocytes and to a lesser degree in renal tubular cells, associated with liver injury (PMID: 15653098). Levels of FABP1 was elevated in patients with hepatocyte injury secondary to alcohol or drug toxicity (PMID: 14563446).