CoraLite® Plus 488-conjugated FABP1 Monoclonal antibody

FABP1 Monoclonal Antibody for FC (Intra), IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig, mouse, rat

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1B6C8

Cat no : CL488-68227

Synonyms

FABP1, FABPL, Fatty acid binding protein 1, L FABP

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Validation Data Gallery

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  IF Staining of mouse small intestine using CL488-68227

  Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using CoraLite® Plus 488 FABP1 antibody (CL488-68227, Clone: 1B6C8 ) at dilution of 1:200.

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  FC experiment of HepG2 using CL488-68227

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human FABP1 (CL488-68227, Clone:1B6C8) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF detected in: mouse small intestine tissue
• Positive FC detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68227 targets FABP1 in IF, FC (Intra) applications and shows reactivity with Human, pig, mouse, rat samples.

• Tested Reactivity: Human, pig, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: FABP1 fusion protein Ag32836
• Full Name: fatty acid binding protein 1, liver
• Calculated Molecular Weight: 127 aa, 14 kDa
• Observed Molecular Weight: 14 kDa
• GenBank Accession Number: BC032801
• Gene Symbol: FABP1
• Gene ID (NCBI): 2168
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FABP1, liver fatty acid-binding protein, is abundant in cytoplasm that regulates lipid transport and metabolism. It plays a role in lipoprotein-mediated cholesterol uptake in hepatocytes (PMID:25732850). FABP1 is mainly expressed in hepatocytes, enterocytes and to a lesser degree in renal tubular cells, associated with liver injury (PMID: 15653098). Levels of FABP1 was elevated in patients with hepatocyte injury secondary to alcohol or drug toxicity (PMID: 14563446).