FABP3 Recombinant monoclonal antibody

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  WB analysis using 86511-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86511-3-RR (FABP3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human skeletal muscle using 86511-3-RR

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 86511-3-RR (FABP3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skeletal muscle using 86511-3-RR

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 86511-3-RR (FABP3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 86511-3-RR

  Biolayer interferometry (BLl) kinetic assays of 86511-3-RR against Human FABP3 were performed. The affinity constant is 1.58 nM.

FABP3 Uni-rAb^® Recombinant Antibody for WB, IHC, ELISA

Cat No. 86511-3-RR

Clone No.251339C10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, ELISA

Synonyms

Fatty acid binding protein 3, Fatty acid-binding protein 3, Fatty acid-binding protein, heart, Heart-type fatty acid-binding protein, H-FABP

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Tested Applications

• Positive WB detected in: human skeletal muscle tissue, mouse skeletal muscle tissue, human heart tissue, mouse heart tissue, rat heart tissue, pig heart tissue
• Positive IHC detected in: human skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86511-3-RR targets FABP3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag1069

  Product name: Recombinant human FABP3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-133 aa of BC007021

  Sequence: MVDAFLGTWKLVDSKNFDDYMKSLGVGFATRQVASMTKPTTIIEKNGDILTLKTHSTFKNTEISFKLGVEFDETTADDRKVKSIVTLDGGKLVHLQKWDGQETTLVRELIDGKLILTLTHGTAVCTRTYEKEA

• Full Name: fatty acid binding protein 3, muscle and heart (mammary-derived growth inhibitor)
• Calculated Molecular Weight: 15 kDa
• Observed Molecular Weight: 15 kDa
• GenBank Accession Number: BC007021
• Gene Symbol: FABP3
• Gene ID (NCBI): 2170
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P05413
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FABP3 (fatty-acid-binding protein 3), also known as heart-type FABP or mammary-derived growth inhibitor (MDGI), is a small 15-kDa cytoplasmic protein transporting fatty acids and other lipophilic substances from the cytoplasm to the nucleus. It is most ubiquitously expressed in heart and skeletal muscle.